Publication: Single-molecule analysis of fluorescently labeled G-protein-coupled receptors reveals complexes with distinct dynamics and organization
Issued Date
2013-01-08
Resource Type
ISSN
10916490
00278424
00278424
Other identifier(s)
2-s2.0-84872195772
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Mahidol University
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SCOPUS
Bibliographic Citation
Proceedings of the National Academy of Sciences of the United States of America. Vol.110, No.2 (2013), 743-748
Suggested Citation
Davide Calebiro, Finn Rieken, Julia Wagner, Titiwat Sungkaworn, Ulrike Zabel, Alfio Borzi, Emanuele Cocucci, Alexander Zürn, Martin J. Lohse Single-molecule analysis of fluorescently labeled G-protein-coupled receptors reveals complexes with distinct dynamics and organization. Proceedings of the National Academy of Sciences of the United States of America. Vol.110, No.2 (2013), 743-748. doi:10.1073/pnas.1205798110 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/32833
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Title
Single-molecule analysis of fluorescently labeled G-protein-coupled receptors reveals complexes with distinct dynamics and organization
Abstract
G-protein-coupled receptors (GPCRs) constitute the largest family of receptors and major pharmacological targets. Whereas many GPCRs have been shown to form di-/oligomers, the size and stability of such complexes under physiological conditions are largely unknown. Here, we used direct receptor labeling with SNAP-tags and total internal reflection fluorescence microscopy to dynamically monitor single receptors on intact cells and thus compare the spatial arrangement, mobility, and supramolecular organization of three prototypical GPCRs: the β1-adrenergic receptor (β1AR), the β2-adrenergic receptor (β2AR), and the γ-aminobutyric acid (GABAB) receptor. These GPCRs showed very different degrees of di-/oligomerization, lowest for β1ARs (monomers/dimers) and highest for GABABreceptors (prevalently dimers/tetramers of heterodimers). The size of receptor complexes increased with receptor density as a result of transient receptor-receptor interactions. Whereas β1-/ β2ARs were apparently freely diffusing on the cell surface, GABABreceptors were prevalently organized into ordered arrays, via interaction with the actin cytoskeleton. Agonist stimulation did not alter receptor di-/oligomerization, but increased the mobility of GABABreceptor complexes. These data provide a spatiotemporal characterization of β1-/β2ARs and GABABreceptors at single-molecule resolution. The results suggest that GPCRs are present on the cell surface in a dynamic equilibrium, with constant formation and dissociation of new receptor complexes that can be targeted, in a ligand-regulated manner, to different cell-surface microdomains.