Publication: The in vivo and in vitro effects of caffeine on rat immune cells activities: B, T and NK cells
Issued Date
1990-12-01
Resource Type
ISSN
0125877X
Other identifier(s)
2-s2.0-0025672709
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Asian Pacific Journal of Allergy and Immunology. Vol.8, No.2 (1990), 77-82
Suggested Citation
D. Kantamala, M. Vongsakul, J. Satayavivad The in vivo and in vitro effects of caffeine on rat immune cells activities: B, T and NK cells. Asian Pacific Journal of Allergy and Immunology. Vol.8, No.2 (1990), 77-82. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/15980
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Title
The in vivo and in vitro effects of caffeine on rat immune cells activities: B, T and NK cells
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Abstract
The effect of caffeine (naturally occurring plant methylxanthine) on immunological cell activities in Sprague-Dawley rat both in vivo and in vitro was studied. A cytotoxic assay was done to study natural killer (NK) cells and a proliferation assay was performed for T and B cell activities. Three different doses of caffeine i.e., 2, 6 and 18 mg/kg/day were administered chronically to Sprague-Dawley rats to assess the effects in vivo. Both NK cell cytotoxicity and B cell proliferative response to pokeweed mitogen (PWM) showed significant decreases (P < 0.05) in rats treated with 6 mg/kg/day, whereas the T cell proliferative response to phytohemagglutinin-P (PHA-P) was significantly increased (P < 0.05) in the rats treated with 18 mg/kg/day. In vitro, caffeine significantly decreases (P < 0.05) B and T cell proliferative responses to PWM and PHA-P at added caffeine concentrations of 10, 20 and 40 μg/ml. However, no effect was observed on NK cells activity. Furthermore, in vitro, a broader dose range of caffeine (1, 10, 100 and 1,000 μg/ml) exhibited dose-dependent inhibition on both B and T cell proliferative responses.
