Publication: Using double-stranded RNA for the control of Laem-Singh Virus (LSNV) in Thai P. monodon
Issued Date
2013-04-15
Resource Type
ISSN
18734863
01681656
01681656
Other identifier(s)
2-s2.0-84875616778
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Biotechnology. Vol.164, No.4 (2013), 449-453
Suggested Citation
Vanvimon Saksmerprome, Thitiporn Thammasorn, Sarocha Jitrakorn, Somjai Wongtripop, Suparerk Borwornpinyo, Boonsirm Withyachumnarnkul Using double-stranded RNA for the control of Laem-Singh Virus (LSNV) in Thai P. monodon. Journal of Biotechnology. Vol.164, No.4 (2013), 449-453. doi:10.1016/j.jbiotec.2013.01.028 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/31328
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Title
Using double-stranded RNA for the control of Laem-Singh Virus (LSNV) in Thai P. monodon
Abstract
Viral inhibition by double-stranded (ds)RNA is a potential therapeutic approach for controlling shrimp viral diseases. Here, we describe the successful oral application of dsRNA targeting Laem-Singh Virus (LSNV) to diminish monodon slow growth syndrome (MSGS) in Thai Penaeus monodon. Shrimp feed formulated with bacterially expressed LSNV-dsRNA was given to shrimp for 9 weeks. RT-PCR results revealed that all control shrimp were LSNV-positive at the end of experiment, while the shrimp that received dsRNA-feed exhibited 20-60% LSNV reduction. The average body weight of treated shrimp (number of shrimp= 100) was significantly higher than that of the control group. Such increase is likely due to the elimination of MSGS caused by LSNV, as size variation of the treated group is much lower than that in the control group. This study demonstrates for the first time that feed with LSNV-specific dsRNA promotes the overall growth of P. monodon and relieves MSGS condition in LSNV-infected shrimp. The work reaffirms the potential of dsRNA application for controlling viral disease in shrimp farming. © 2013 Elsevier B.V.