Study of hematopoietic progenitor cells, hematological values and lymphocyte subsets in cord blood: Application for cord blood transplantation

Abstract

Hematological values, lymphocyte subsets and hematopoietic progenitor cells from normal term cord blood samples were studied, compared with normal adult blood, and analysed to determine whether a single collection of cord blood is sufficient for transplantation in adults. The parameters were assayed by automatic cells counter, flow cytometry and semisolid cell culture. All of the hematological values except RBC and MCHC were higher than in normal adult blood. Sex had an influence on RBC, Hb, Hct, Pit and reticulocyte counts. For lymphocyte subsets, all of the absolute CD3+, CD4+, CD8+ counts and T helper: suppressor ratio were higher than those of adult blood. All of the hematopoietic progenitor cells in cord blood were also higher than in adult blood. The mean volume of cord blood for each collection was 80.75±4.81 ml and the mean numbers of nucleated cells, CFU-GM and CD34+ were 13.51±0.38×108 cells, 4.33±0.66×105 colonies and 42.65±7.00×105 cells respectively. This 80 ml of cord blood would contain sufficient marrow repopulating cells fer a recipient weighing about 20 kg. Recently developed technology, including ex vivo expansion may even permit transplants in adults.