Publication: Approach toward enhancement of halophilic protease production by Halobacterium sp. strain LBU50301 using statistical design response surface methodology
Issued Date
2016-06-01
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2215017X
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2-s2.0-84963522303
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Mahidol University
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SCOPUS
Bibliographic Citation
Biotechnology Reports. Vol.10, (2016), 17-28
Suggested Citation
Julalak Chuprom, Preeyanuch Bovornreungroj, Mehraj Ahmad, Duangporn Kantachote, Sawitree Dueramae Approach toward enhancement of halophilic protease production by Halobacterium sp. strain LBU50301 using statistical design response surface methodology. Biotechnology Reports. Vol.10, (2016), 17-28. doi:10.1016/j.btre.2016.02.004 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/43008
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Title
Approach toward enhancement of halophilic protease production by Halobacterium sp. strain LBU50301 using statistical design response surface methodology
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Abstract
© 2016 The Author. Published by Elsevier B.V. A new potent halophilic protease producer, Halobacterium sp. strain LBU50301 was isolated from salt-fermented fish samples (budu) and identified by phenotypic analysis, and 16S rDNA gene sequencing. Thereafter, sequential statistical strategy was used to optimize halophilic protease production from Halobacterium sp. strain LBU50301 by shake-flask fermentation. The classical one-factor-at-a-time (OFAT) approach determined gelatin was the best nitrogen source. Based on Plackett-Burman (PB) experimental design; gelatin, MgSO4·7H2O, NaCl and pH significantly influenced the halophilic protease production. Central composite design (CCD) determined the optimum level of medium components. Subsequently, an 8.78-fold increase in corresponding halophilic protease yield (156.22 U/mL) was obtained, compared with that produced in the original medium (17.80 U/mL). Validation experiments proved the adequacy and accuracy of model, and the results showed the predicted value agreed well with the experimental values. An overall 13-fold increase in halophilic protease yield was achieved using a 3 L laboratory fermenter and optimized medium (231.33 U/mL).