Publication: Xenorhabdus thuongxuanensis sp. nov. and Xenorhabdus eapokensis sp. nov., isolated from Steinernema species
Issued Date
2017-05-01
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14665026
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2-s2.0-85020441413
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Mahidol University
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SCOPUS
Bibliographic Citation
International Journal of Systematic and Evolutionary Microbiology. Vol.67, No.5 (2017), 1107-1114
Suggested Citation
Peter Kämpfer, Nicholas J. Tobias, Long Phan Ke, Helge B. Bode, Stefanie P. Glaeser Xenorhabdus thuongxuanensis sp. nov. and Xenorhabdus eapokensis sp. nov., isolated from Steinernema species. International Journal of Systematic and Evolutionary Microbiology. Vol.67, No.5 (2017), 1107-1114. doi:10.1099/ijsem.0.001770 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/41480
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Title
Xenorhabdus thuongxuanensis sp. nov. and Xenorhabdus eapokensis sp. nov., isolated from Steinernema species
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Abstract
© 2017 IUMS. Two slightly yellowish-pigmented, oxidase-negative, rod-shaped and Gram-stain-negative bacterial strains (30TX1Tand DL20T), isolated from Steinernema sangi and Steinernema eapokense, respectively, during soil sampling in Vietnam were studied using a polyphasic taxonomic approach. Strain 30TX1Tshowed highest 16S rRNA gene sequence similarity to the type strain of Xenorhabdus ehlersii (98.9%) and strain DL20Tto that of Xenorhabdus ishibashii (98.7%). Sequence similarities to all other Xenorhabdus species were lower (<98.4%). The two strains shared 98% 16S rRNA gene sequence similarity. Multilocus sequence analysis (MLSA) based on concatenated partial recA, dnaN, gltX, gyrB and infB gene sequences showed a clear distinction of strains 30TX1Tand DL20Tamong each other and to the closest related type strains. DNA–DNA hybridizations between strain DL20Tand the type strain of X. ishibashii resulted in a relatedness value of 53%. Genome-togenome- based comparisons gave average nucleotide identities of 93.6% (reciprocal 93.5%) for strain 30TX1Tand X. ehlersii DSM 16337T, of 92.8% (reciprocal 93%) for strain DL20Tand X. ishibashii DSM 22670Tand of 93.0% (reciprocal 93.2%) for the two novel strains. The fatty acid profile of the strains consisted of the major fatty acids C14: 0, C16: 0, C17: 0cyclo, C16: 1ω7c and/or iso-C15: 02-OH, and C18: 1ω7c. Genome-to-genome comparison and MLSA results together with the differential biochemical and chemotaxonomic properties showed that strains 30TX1Tand DL20Trepresent novel Xenorhabdus species, for which the names Xenorhabdus thuongxuanensis sp. nov. (type strain 30TX1T=CCM 8727T=LMG 29916T) and Xenorhabdus eapokensis sp. nov. (type strain DL20T=CCM 8728T=LMG 29917T) are proposed, respectively.