Publication: Glucan from spent brewer's yeast: Preparation, analysis and use as a potential immunostimulant in shrimp feed
Issued Date
2002-01-01
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ISSN
09593993
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2-s2.0-0036333131
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Mahidol University
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SCOPUS
Bibliographic Citation
World Journal of Microbiology and Biotechnology. Vol.18, No.6 (2002), 527-539
Suggested Citation
P. Thanardkit, P. Khunrae, M. Suphantharika, C. Verduyn Glucan from spent brewer's yeast: Preparation, analysis and use as a potential immunostimulant in shrimp feed. World Journal of Microbiology and Biotechnology. Vol.18, No.6 (2002), 527-539. doi:10.1023/A:1016322227535 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/20094
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Title
Glucan from spent brewer's yeast: Preparation, analysis and use as a potential immunostimulant in shrimp feed
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Abstract
Spent brewer's yeast was autolysed and the residual solids were further treated with hot alkali to produce a fraction containing 95% (w/w) carbohydrate, with a glycogen content of 42% and an apparent glucan content of ca. 53% (w/w). FTIR and solid state C-NMR spectra basically showed a spectrum characteristic for an impure β-glucan. In vitro, this glucan induced release of prophenoloxidase from intact black tiger shrimp haemocytes. When fed to shrimp at 0.2% (w/w of the feed) for 3 days, significant increases in phenol oxidase, the number of haemocytes and the bacterial killing activity against the pathogen Vibrio harveyi were observed. Furthermore, this feeding resulted in the appearance of an inducible β-(1, 3)-glucanase in the liver. Methods to analyse the fractions are discussed and a number of commercial products with potential immune stimulating properties have been included for comparative purposes.