Publication: Synthesis and Transfection Efficiencies of Divalent Ammonium Headgroup Cationic Lipids with Different Hydrophobic Tails
Issued Date
2020-05-01
Resource Type
ISSN
1608330X
10681620
10681620
Other identifier(s)
2-s2.0-85087058638
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Mahidol University
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SCOPUS
Bibliographic Citation
Russian Journal of Bioorganic Chemistry. Vol.46, No.3 (2020), 417-428
Suggested Citation
W. Radchatawedchakoon, N. Niyomtham, C. Thongbamrer, C. Posa, U. Sakee, W. Roobsoong, J. Sattabongkot, P. Opanasopit, B. Yingyongnarongkul Synthesis and Transfection Efficiencies of Divalent Ammonium Headgroup Cationic Lipids with Different Hydrophobic Tails. Russian Journal of Bioorganic Chemistry. Vol.46, No.3 (2020), 417-428. doi:10.1134/S1068162020030140 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/57738
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Title
Synthesis and Transfection Efficiencies of Divalent Ammonium Headgroup Cationic Lipids with Different Hydrophobic Tails
Abstract
© 2020, Pleiades Publishing, Ltd. Abstract: Cationic lipids with amide or carbamate linker and divalent cationic headgroups were synthesized for a transfection study. Sixteen cationic lipids with double acyl or cholesteryl hydrophobic tails and divalent ammonium headgroups were obtained by a solid phase methodology. The structures of the synthesized lipids were characterized by spectroscopic techniques. The DNA binding affinity of the synthetic lipids was confirmed by gel electrophoresis technique. A lipid with non-symmetrical in length hydrophobic tails (lauroyl and stearoyl) demonstrated higher transfection efficiency than the other lipids in the presence of the helper lipid, dioleoylphosphatidylethanolamine (DOPE), when optimized into the HEK293 cell. At 20% of serum, the same lipid showed relative transfection efficiency equal to that of LipofectamineTM 2000 (L2K). The transfection efficiency of the active lipid was also studied with HeLa, PC3, and HC-04 cell lines. Concerning cell viability, the most potent lipid was non-toxic with respect to the HEK293 and HC-04 cells (over 85% survival). The lipid formed particles of around 380 nm in size with zeta potential around 35 mV at 1: 30 DNA/lipid (weight/weight) ratio.