Publication: Rat sperm AS-A: Subcellular localization in testis and epididymis and surface distribution in epididymal sperm
Issued Date
2004-11-01
Resource Type
ISSN
0302766X
Other identifier(s)
2-s2.0-8644232435
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Cell and Tissue Research. Vol.318, No.2 (2004), 353-363
Suggested Citation
Apichart Ngernsoungnern, Wattana Weerachatyanukul, Arpornrad Saewu, Siriporn Thitilertdecha, Prasert Sobhon, Prapee Sretarugsa Rat sperm AS-A: Subcellular localization in testis and epididymis and surface distribution in epididymal sperm. Cell and Tissue Research. Vol.318, No.2 (2004), 353-363. doi:10.1007/s00441-004-0985-4 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/21126
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Rat sperm AS-A: Subcellular localization in testis and epididymis and surface distribution in epididymal sperm
Other Contributor(s)
Abstract
In this study, we investigated the subcellular compartmentalization of arylsulfatase-A (AS-A) in the testis and epididymis as well as the surface distribution in rat epididymal sperm. Testicular AS-A was compartmentalized specifically to the area underneath the outer acrosomal membrane of the acrosomal granule and to the dorsal aspect of the sperm acrosome. Epididymal AS-A was synthesized in the endoplasmic reticular (ER) network of principal cells and secreted into epididymal lumen as evident by its reactivity in the apical cytoplasm and vesicles therein underneath stereocilia. In clear cells, AS-A reactivity was found throughout the cytoplasmic machineries involved in endocytosis. Surface distribution of AS-A was initially detectable at the concave ridge as early as in sperm of the initial segment (IS). AS-A was additionally localized to the post-acrosomal region in caput (CP), corpus (CO) and cauda (CD) epididymal sperm. The expression levels of surface AS-A gradually increased during sperm transit from IS to CD epididymidis. These results favored the adsorption of AS-A from epididymal fluid onto the sperm surface, rather than shunting from the acrosome as a consequence of capacitation- associated membrane priming. © Springer-Verlag 2004.