Publication: Development of multiplex real-time polymerase chain reaction for detection of Entamoeba histolytica, Entamoeba dispar, and Entamoeba moshkovskii in clinical specimens
Issued Date
2010-10-01
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ISSN
00029637
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2-s2.0-79958061530
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Mahidol University
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SCOPUS
Bibliographic Citation
American Journal of Tropical Medicine and Hygiene. Vol.83, No.4 (2010), 909-913
Suggested Citation
Zulhainan Hamzah, Songsak Petmitr, Mathirut Mungthin, Saovanee Leelayoova, Porntip Chavalitshewinkoon-Petmitr Development of multiplex real-time polymerase chain reaction for detection of Entamoeba histolytica, Entamoeba dispar, and Entamoeba moshkovskii in clinical specimens. American Journal of Tropical Medicine and Hygiene. Vol.83, No.4 (2010), 909-913. doi:10.4269/ajtmh.2010.10-0050 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/29191
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Title
Development of multiplex real-time polymerase chain reaction for detection of Entamoeba histolytica, Entamoeba dispar, and Entamoeba moshkovskii in clinical specimens
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Abstract
Multiplex real-time polymerase chain reaction (PCR) was developed for differential detection of Entamoeba histolytica, Entamoeba dispar, and Entamoeba moshkovskii. Specific primers were designed for all three species, and then differentiation of E. histolytica and E. dispar was achieved simultaneously using a hybridization probe and melting curve analysis, whereas E. moshkovskii was detected with a separate probe under the same condition. This assay detected as little as 0.2 pg of E. histolytica DNA and 2 pg each for E. dispar and E. moshkovskii DNA. Thirty-five clinical samples suspected to be E. histolytica infection by microscopy were tested. The results showed 32 positive samples; four samples were E. histolytica and 28 samples were E. dispar. Interestingly, one E. dispar positive sample showed a mixed infection with E. moshkovskii. This is the first report of E. moshkovskii infection from Thailand and this assay is currently the most rapid and sensitive method to differentiate these human amoebas. Copyright © 2010 by The American Society of Tropical Medicine and Hygiene.