Publication: Ultrastructural studies on dengue virus infection of human lymphoblasts
1
Issued Date
1978-12-01
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ISSN
00199567
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2-s2.0-0018097695
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Mahidol University
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SCOPUS
Bibliographic Citation
Infection and Immunity. Vol.20, No.1 (1978), 173-179
Suggested Citation
S. Sriurairatna, N. Bhamarapravati, A. R. Diwan, S. B. Halstead Ultrastructural studies on dengue virus infection of human lymphoblasts. Infection and Immunity. Vol.20, No.1 (1978), 173-179. Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/13067
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Title
Ultrastructural studies on dengue virus infection of human lymphoblasts
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Abstract
Ultrastructural studies of dengue-2 virus-infected lymphoblastoid Raji cells showed that the virus induced an increase in the size of the rough endoplasmic reticula (RER) and that the replication of the virus was confined to the cisternae of these RER. The proliferating RER formed cytoplasmic inclusions that could be seen by light microscopy. This observation could be used as evidence of a cytopathogenic effect of dengue virus on infected Raji cells in routine cultures. Accumulation of virions in the infected cells was minimal in comparison with other cell systems, however. Sporadic clusters of mature virions were often seen on the plasma membrane. These extracellular virions were distributed adjacent to the virus-bearing RER and were presumably released virions. Vertical transmission of the virus was evident in mitotic lymphoblasts. The replication pattern of dengue virus in lymphoblastoid cells suggest that efforts should be made to determine whether blast-transformed lymphocytes, numerous in secondary dengue infections, support dengue virus replication in vivo.