Publication: Genomic investigation of a suspected Klebsiella pneumoniae outbreak in a neonatal care unit in sub-Saharan Africa
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Issued Date
2021-01-01
Resource Type
ISSN
20575858
Other identifier(s)
2-s2.0-85121993914
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Mahidol University
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SCOPUS
Bibliographic Citation
Microbial Genomics. Vol.7, No.11 (2021)
Suggested Citation
Jennifer Cornick, Patrick Musicha, Chikondi Peno, Ezgi Seager, Pui Ying Iroh Tam, Sithembile Bilima, Aisleen Bennett, Neil Kennedy, Nicholas Feasey, Eva Heinz, Amy K. Cain Genomic investigation of a suspected Klebsiella pneumoniae outbreak in a neonatal care unit in sub-Saharan Africa. Microbial Genomics. Vol.7, No.11 (2021). doi:10.1099/mgen.0.000703 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/76317
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Title
Genomic investigation of a suspected Klebsiella pneumoniae outbreak in a neonatal care unit in sub-Saharan Africa
Other Contributor(s)
Malawi-Liverpool-Wellcome Trust Clinical Research Programme
University of Malawi College of Medicine
Queen's University Belfast
St George’s, University of London
Heartlands Hospital
Liverpool School of Tropical Medicine
University of Liverpool
Macquarie University
Mahidol University
University of Edinburgh, College of Medicine and Veterinary Medicine
Nuffield Department of Medicine
Wellcome Sanger Institute
University of Malawi College of Medicine
Queen's University Belfast
St George’s, University of London
Heartlands Hospital
Liverpool School of Tropical Medicine
University of Liverpool
Macquarie University
Mahidol University
University of Edinburgh, College of Medicine and Veterinary Medicine
Nuffield Department of Medicine
Wellcome Sanger Institute
Abstract
A special-care neonatal unit from a large public hospital in Malawi was noted as having more frequent, difficult-to-treat infec-tions, and a suspected outbreak of multi-drug-resistant Klebsiella pneumoniae was investigated using genomic characterisa-tion. All K. pneumoniae bloodstream infections (BSIs) from patients in the neonatal ward (n=62), and a subset of K. pneumoniae BSI isolates (n=38) from other paediatric wards in the hospital, collected over a 4 year period were studied. After whole genome sequencing, the strain sequence types (STs), plasmid types, virulence and resistance genes were identified. One ST340 clone, part of clonal complex 258 (CC258) and an ST that drives hospital outbreaks worldwide, harbouring numerous resistance genes and plasmids, was implicated as the likely cause of the outbreak. This study contributes molecular information necessary for tracking and characterizing this important hospital pathogen in sub-Saharan Africa.