Publication:
Robust continuous in vitro culture of the Plasmodium cynomolgi erythrocytic stages

Issued Date

2019-12-01

Resource Type

Article

ISSN

20411723

DOI

10.1038/s41467-019-11332-4

Other identifier(s)

2-s2.0-85070687044

Rights

Mahidol University

Rights Holder(s)

SCOPUS

Bibliographic Citation

Nature Communications. Vol.10, No.1 (2019)

Suggested Citation

Adeline C.Y. Chua, Jessica Jie Ying Ong, Benoit Malleret, Rossarin Suwanarusk, Varakorn Kosaisavee, Anne Marie Zeeman, Caitlin A. Cooper, Kevin S.W. Tan, Rou Zhang, Bee Huat Tan, Siti Nurdiana Abas, Andy Yip, Anne Elliot, Chester J. Joyner, Jee Sun Cho, Kate Breyer, Szczepan Baran, Amber Lange, Steven P. Maher, François Nosten, Christophe Bodenreider, Bryan K.S. Yeung, Dominique Mazier, Mary R. Galinski, Nathalie Dereuddre-Bosquet, Roger Le Grand, Clemens H.M. Kocken, Laurent Rénia, Dennis E. Kyle, Thierry T. Diagana, Georges Snounou, Bruce Russell, Pablo Bifani Robust continuous in vitro culture of the Plasmodium cynomolgi erythrocytic stages. Nature Communications. Vol.10, No.1 (2019). doi:10.1038/s41467-019-11332-4 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/50025

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Title

Robust continuous in vitro culture of the Plasmodium cynomolgi erythrocytic stages

Author(s)

Adeline C.Y. Chua
 Jessica Jie Ying Ong
 Benoit Malleret
 Rossarin Suwanarusk
 Varakorn Kosaisavee
 Anne Marie Zeeman
 Caitlin A. Cooper
 Kevin S.W. Tan
 Rou Zhang
 Bee Huat Tan
 Siti Nurdiana Abas
 Andy Yip
 Anne Elliot
 Chester J. Joyner
 Jee Sun Cho
 Kate Breyer
 Szczepan Baran
 Amber Lange
 Steven P. Maher
 François Nosten
 Christophe Bodenreider
 Bryan K.S. Yeung
 Dominique Mazier
 Mary R. Galinski
 Nathalie Dereuddre-Bosquet
 Roger Le Grand
 Clemens H.M. Kocken
 Laurent Rénia
 Dennis E. Kyle
 Thierry T. Diagana
 Georges Snounou
 Bruce Russell
 Pablo Bifani

Other Contributor(s)

A-Star, Singapore Immunology Network
 Centre de Recherche en Immunologie des Infections Virales et des Maladies Auto-Immunes
 London School of Hygiene & Tropical Medicine
 The University of Georgia
 Yong Loo Lin School of Medicine
 Novartis Institute for Tropical Diseases Pte. Ltd.
 Biomedical Primate Research Centre - Rijswijk
 University of Otago
 Mahidol University
 Nuffield Department of Clinical Medicine
 Sorbonne Universite
 Emory University
 Inserm
 Novartis Institutes for BioMedical Research

Abstract

© 2019, The Author(s). The ability to culture pathogenic organisms substantially enhances the quest for fundamental knowledge and the development of vaccines and drugs. Thus, the elaboration of a protocol for the in vitro cultivation of the erythrocytic stages of Plasmodium falciparum revolutionized research on this important parasite. However, for P. vivax, the most widely distributed and difficult to treat malaria parasite, a strict preference for reticulocytes thwarts efforts to maintain it in vitro. Cultivation of P. cynomolgi, a macaque-infecting species phylogenetically close to P. vivax, was briefly reported in the early 1980s, but not pursued further. Here, we define the conditions under which P. cynomolgi can be adapted to long term in vitro culture to yield parasites that share many of the morphological and phenotypic features of P. vivax. We further validate the potential of this culture system for high-throughput screening to prime and accelerate anti-P. vivax drug discovery efforts.

Keyword(s)

Biochemistry, Genetics and Molecular Biology
 Chemistry

Availability

URI

https://repository.li.mahidol.ac.th/handle/20.500.14594/50025

Collections

Scopus 2019