Publication: Negative-ELISA using native and filtrated cystic fluid antigens to rule out cystic echinococcosis.
Issued Date
2005-12-01
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ISSN
01251562
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2-s2.0-33646191306
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Mahidol University
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SCOPUS
Bibliographic Citation
The Southeast Asian journal of tropical medicine and public health.. Vol.36 Suppl 4, (2005), 139-145
Suggested Citation
Paron Dekumyoy, Doungrat Riyong, Wallop Pakdee, Jitra Waikagul Negative-ELISA using native and filtrated cystic fluid antigens to rule out cystic echinococcosis.. The Southeast Asian journal of tropical medicine and public health.. Vol.36 Suppl 4, (2005), 139-145. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/16655
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Title
Negative-ELISA using native and filtrated cystic fluid antigens to rule out cystic echinococcosis.
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Abstract
An increasing number of cases of echinococcosis in Thailand have been imported, probably native infections and medical transfers. Serodiagnosis is one diagnostic choice for interpreting infections before a further step is done. Due to limited antigen, indirect ELISA has been used as a negative screening test for IgG-detection to rule out echinococcosis. Native hydatid cystic fluid (HCF) antigen from Belgium was used for such testing, in which the ODs-ELISA of samples were compared with those of two positive controls. Subsequently, hydatid cyst fluid from a Thai patient was obtained and the filtrated cyst fluid antigen [(<30)-(>10) kDa, HCF30.10] was prepared to develop negative screening results for the serum samples. By using HCF, three of twenty-four samples resulted in higher ODs-ELISA than the controls. In an attempt to observe the cross-reactivity of this native antigen, IgG-antibodies from many helminthiases cross-reacted and showed high ODs-ELISA. The HCF30.10 Ag was used to develop the test and analyze IgG-antibodies from 5 positive controls (2 parasite-confirmed and 3 positive-serodiagnosed), 183 heterologous cases of 29 diseases and 50 healthy control sera. At a cut-off value of 0.484, the test had 100% sensitivity and 42% specificity. Only Malayan filariasis, onchocercosis, fascioliasis, amebiasis, giardiasis and blastocystosis gave true negatives. Antibodies from nematodiases strongly cross-reacted with HCF30.10 Ag. Nine of fifty (18%) healthy serum controls produced higher OD-values than the cut-off. The routine ELISA uses the HCF30.10 Ag to produce a negative result to echinococcosis, because limited cystic fluid antigen (Thai patient) for test improvement, a lot of cross-reactions and only two protoscolex-positive controls are available.