Publication: Identification of uncommon pathogenic bacteria by MALDI-TOF mass spectrometry using a custom library of siriraj hospital
Issued Date
2018-03-01
Resource Type
ISSN
22288082
Other identifier(s)
2-s2.0-85051599308
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Siriraj Medical Journal. Vol.70, No.2 (2018), 127-130
Suggested Citation
Kanda Ekcharoenkul, Popchai Ngamskulrungroj, Keerati Joyjamras, Amornrut Leelaporn, Azian Harun, Pattarachai Kiratisin Identification of uncommon pathogenic bacteria by MALDI-TOF mass spectrometry using a custom library of siriraj hospital. Siriraj Medical Journal. Vol.70, No.2 (2018), 127-130. doi:10.14456/smj.2018.21 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/46901
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Identification of uncommon pathogenic bacteria by MALDI-TOF mass spectrometry using a custom library of siriraj hospital
Abstract
© 2018, Faculty of Medicine Siriraj Hospital, Mahidol University. Objective: Matrix Assisted Laser Desorption Ionization Time-Of-Flight mass spectrometry (MALDI-TOF MS) is a rapid and cost-effective technique that serves as an alternative method for bacterial identification in microbiology laboratories. This study aimed to determine the performance of MALDI-TOF MS for identifying uncommon and/ or fastidious bacteria comparing with automated identification method. Methods: We observed the performances of original and extended databases after adding of in-house MALDI-TOF MS database for a total of 54 isolates belonging to 16 genera of uncommon and/or fastidious bacteria. Results: The extended database improved identification of these bacteria significantly except for Listeria monocytogenes and Elizabethkingia meningoseptica. Conclusion: This study provides a possibility for identification of uncommon and/or fastidious bacteria by MALDITOF MS method.