Publication: Oxidized Carbon Black: Preparation, Characterization and Application in Antibody Delivery across Cell Membrane
Issued Date
2018-12-01
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ISSN
20452322
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2-s2.0-85041696827
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Mahidol University
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SCOPUS
Bibliographic Citation
Scientific Reports. Vol.8, No.1 (2018)
Suggested Citation
Kittima Amornwachirabodee, Nattapol Tantimekin, Porntip Pan-In, Tanapat Palaga, Prompong Pienpinijtham, Chonlatip Pipattanaboon, Thanyada Sukmanee, Patcharee Ritprajak, Promchat Charoenpat, Pannamthip Pitaksajjakul, Pongrama Ramasoota, Supason Wanichwecharungruang Oxidized Carbon Black: Preparation, Characterization and Application in Antibody Delivery across Cell Membrane. Scientific Reports. Vol.8, No.1 (2018). doi:10.1038/s41598-018-20650-4 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/47471
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Title
Oxidized Carbon Black: Preparation, Characterization and Application in Antibody Delivery across Cell Membrane
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Abstract
© 2018 The Author(s). Modulating biomolecular networks in cells with peptides and proteins has become a promising therapeutic strategy and effective biological tools. A simple and effective reagent that can bring functional proteins into cells can increase efficacy and allow more investigations. Here we show that the relatively non-toxic and non-immunogenic oxidized carbon black particles (OCBs) prepared from commercially available carbon black can deliver a 300 kDa protein directly into cells, without an involvement of a cellular endocytosis. Experiments with cell-sized liposomes indicate that OCBs directly interact with phospholipids and induce membrane leakages. Delivery of human monoclonal antibodies (HuMAbs, 150 kDa) with specific affinity towards dengue viruses (DENV) into DENV-infected Vero cells by OCBs results in HuMAbs distribution all over cells' interior and effective viral neutralization. An ability of OCBs to deliver big functional/therapeutic proteins into cells should open doors for more protein drug investigations and new levels of antibody therapies and biological studies.