Publication: Nitric oxide modulates human chorionic gonadotropin-induced ovulation in the rabbit
Issued Date
1997-01-01
Resource Type
ISSN
00150282
Other identifier(s)
2-s2.0-0031028698
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Mahidol University
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SCOPUS
Bibliographic Citation
Fertility and Sterility. Vol.67, No.3 (1997), 548-552
Suggested Citation
John S. Hesla, Sangchai Preutthipan, Michael P. Maguire, Thomas S.K. Chang, Edward E. Wallach, Arunasalam M. Dharmarajan Nitric oxide modulates human chorionic gonadotropin-induced ovulation in the rabbit. Fertility and Sterility. Vol.67, No.3 (1997), 548-552. doi:10.1016/S0015-0282(97)80084-2 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/18214
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Title
Nitric oxide modulates human chorionic gonadotropin-induced ovulation in the rabbit
Abstract
Objective: To examine the potential role of the L-arginine:nitric oxide pathway in hCG-induced ovulation in the rabbit. Design: Randomized, controlled animal study. Setting: University research laboratory. Intervention(s): Nitric oxide synthase, the enzyme that produces nitric oxide (NO), was immunohistochemically localized in the ovary. N(G)-nitro-L- arginine methyl ester (L-NAME), an analogue of L-arginine, which inhibits the enzyme NO synthase, and the inactive D-enantiomer were administered in vivo and/or in vitro via an isolated, perfused ovary preparation during the periovulatory period. Main Outcome Measure(s): Rate of follicular rupture/ovulatory efficiency). Result(s): Immunohistochemical staining for NO synthase was localized specifically to the granulosa cell layer of the follicle and the endothelium and adventitia of ovarian blood vessels. In vivo administration of L-NAME significantly reduced the percentage of large follicles that ovulated in response to hCG (treated 24.6%, control 68.1%). Similarly, exposure of the in vitro-perfused ovary to L-NAME significantly reduced follicular rupture (treated 32.8%, control 64.2%). In contrast, addition of an equimolar concentration of D-NAME to the perfusion medium had no significant effect on the rate of ovulation (treated 83.3%, control 61.3%). Conclusion(s): The stereospecific inhibition of follicular rupture by the arginine analogue suggests that NO production by the ovary is an important feature of the normal physiologic processes of the periovulatory period.