Publication: Involvement of miR164- and miR167-mediated target gene expressions in responses to water deficit in cassava
| dc.contributor.author | P. Phookaew | en_US |
| dc.contributor.author | S. Netrphan | en_US |
| dc.contributor.author | P. Sojikul | en_US |
| dc.contributor.author | J. Narangajavana | en_US |
| dc.contributor.other | Mahidol University | en_US |
| dc.contributor.other | Thailand National Center for Genetic Engineering and Biotechnology | en_US |
| dc.date.accessioned | 2018-11-09T01:47:16Z | |
| dc.date.available | 2018-11-09T01:47:16Z | |
| dc.date.issued | 2014-01-01 | en_US |
| dc.description.abstract | Cassava (Manihot esculenta Crantz) is an important crop and it is significantly affected by water stress. The computational analysis of cis-regulatory elements in promoter regions of 21 drought-responsive miRNA gene families and 35 miRNA-target genes in cassava indicated some elements relevant to drought stress responses. To investigate the role of miRNAs and target genes in responses to a water deficit in cassava in more detail, in vitro plantlets were subjected to an imitated water deficit by 40 % polyethylene glycol. Using RT-qPCR, the differential expression of the cassava miR164/MesNAC and miR167/MesARF6/8 were observed to be associated with changes in the leaf shape, stomatal closure, and relative water content. The modified 5′-RNA ligase-mediated rapid amplification of cDNA-end (5'RLM-RACE) experiment confirmed MesNAC and MesARF8 as the in vivo-target genes of miR164 and miR167, respectively, in cassava leaf. The possible functions of miR164 and miR167-target genes in response to water deficit are discussed. © 2014 Springer Science+Business Media Dordrecht. | en_US |
| dc.identifier.citation | Biologia Plantarum. Vol.58, No.3 (2014), 469-478 | en_US |
| dc.identifier.doi | 10.1007/s10535-014-0410-0 | en_US |
| dc.identifier.issn | 00063134 | en_US |
| dc.identifier.other | 2-s2.0-84906078606 | en_US |
| dc.identifier.uri | https://repository.li.mahidol.ac.th/handle/20.500.14594/33120 | |
| dc.rights | Mahidol University | en_US |
| dc.rights.holder | SCOPUS | en_US |
| dc.source.uri | https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84906078606&origin=inward | en_US |
| dc.subject | Agricultural and Biological Sciences | en_US |
| dc.title | Involvement of miR164- and miR167-mediated target gene expressions in responses to water deficit in cassava | en_US |
| dc.type | Article | en_US |
| dspace.entity.type | Publication | |
| mu.datasource.scopus | https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84906078606&origin=inward | en_US |