Publication: Comparative genomics of Edwardsiella ictaluri revealed four distinct host-specific genotypes and thirteen potential vaccine candidates
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Issued Date
2021-07-01
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ISSN
10898646
08887543
08887543
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2-s2.0-85104906694
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Mahidol University
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SCOPUS
Bibliographic Citation
Genomics. Vol.113, No.4 (2021), 1976-1987
Suggested Citation
Vimbai Irene Machimbirike, Pichahpuk Uthaipaisanwong, Pongsak Khunrae, Ha Thanh Dong, Saengchan Senapin, Triwit Rattanarojpong, Sawannee Sutheeworapong Comparative genomics of Edwardsiella ictaluri revealed four distinct host-specific genotypes and thirteen potential vaccine candidates. Genomics. Vol.113, No.4 (2021), 1976-1987. doi:10.1016/j.ygeno.2021.04.016 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/76128
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Title
Comparative genomics of Edwardsiella ictaluri revealed four distinct host-specific genotypes and thirteen potential vaccine candidates
Abstract
Edwardsiella ictaluri has been considered an important threat for catfish aquaculture industry for more than 4 decades and an emerging pathogen of farmed tilapia but only 9 sequenced genomes were publicly available. We hereby report two new complete genomes of E. ictaluri originated from diseased hybrid red tilapia (Oreochromis sp.) and striped catfish (Pangasianodon hypophthalmus) in Southeast Asia. E. ictaluri species has an open pan-genome consisting of 2615 core genes and 5592 pan genes. Phylogenetic analysis using core genome MLST (cgMLST) and ANI values consistently placed E. ictaluri isolates into 4 host-specific genotypes. Presence of unique genes and absence of certain genes from each genotype provided potential biomarkers for further development of genotyping scheme. Vaccine candidates with high antigenic, solubility and secretion probabilities were identified in silico from the core genes. Microevolution within the species is brought about by bacteriophages and insertion elements and possibly drive host adaptation.