Publication: Serum protein fractions from children of differing nutritional status analysed by polyacrylamide gel electrophoresis and electroimmunoassay
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1976-01-01
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14752662
00071145
00071145
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2-s2.0-0017240199
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Mahidol University
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SCOPUS
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British Journal of Nutrition. Vol.35, No.2 (1976), 211-222
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F. P. Schelp, P. Migasena, S. Saovakontha, Praneet Pongpaew, Venus Supawan Serum protein fractions from children of differing nutritional status analysed by polyacrylamide gel electrophoresis and electroimmunoassay. British Journal of Nutrition. Vol.35, No.2 (1976), 211-222. doi:10.1079/BJN19760025 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/10913
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Serum protein fractions from children of differing nutritional status analysed by polyacrylamide gel electrophoresis and electroimmunoassay
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1. The nutritional status of children showing no clinical signs of malnutrition, from the University School of Khon Kaen, Khon Kaen province, north-east Thailand and from two villages nearby, was tested. The children were grouped according to their body-weight expressed as a percentage of expected weight-for-height (Harvard standards (Stuart & Stevenson, 1959), as given by Jelliffe (1966)). 2. The differing prealbumin concentrations indicated that nutritional status differed between the groups. 3. The urinary urea: creatinine ratio was significantly lower in the village children compared with the children from Khon Kaen, indicative of the higher dietary protein intake of the latter. 4. α 1 -Acid glycoprotein and the first ‘post-albumin peak’ (obtained by polyacrylamide gel electrophoresis of serum and containing mainly Gc-globulin, α 1 -antichymotrypsin and α 1 -B-glycoprotein) were found to be significantly higher in the village children compared with children from Khon Kaen. 5. The three main proteins of the first ‘post-albumin peakk from polyacrylamide gel electrophoresis of serum were tested separately using the electroimmunoassay method. There was no significant difference in Gc-globulin between the children from Khon Kaen and the village children. The concentration of α 1 -B-glycoprotein from those Khon Kaen children whose bodyweight was more than 95% expected weight-for-height was significantly lower compared with that of village children. α 1 -Antichymotrypsin concentration was significantly higher in serum from Khon Kaen children than in serum from village children. © 1976, The Nutrition Society. All rights reserved.