Publication: Ultrasonication extraction and solid phase extraction clean-up for quantification of aristolochic acid i in the thai herbal antitussive 3 formula by high performance liquid chromatography with photodiode array detection
Issued Date
2018-05-01
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22288082
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2-s2.0-85051580061
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Mahidol University
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SCOPUS
Bibliographic Citation
Siriraj Medical Journal. Vol.70, No.3 (2018), 227-232
Suggested Citation
Jantanee Wattanarangsan, Pravit Akarasereenont, Natchagorn Lumlerdkij, Sirikul Chotewuttakorn, Kanokorn Wattanapornchai, Tawee Laohapand Ultrasonication extraction and solid phase extraction clean-up for quantification of aristolochic acid i in the thai herbal antitussive 3 formula by high performance liquid chromatography with photodiode array detection. Siriraj Medical Journal. Vol.70, No.3 (2018), 227-232. doi:10.14456/smj.2018.37 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/46683
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Title
Ultrasonication extraction and solid phase extraction clean-up for quantification of aristolochic acid i in the thai herbal antitussive 3 formula by high performance liquid chromatography with photodiode array detection
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Abstract
© 2018, Faculty of Medicine Siriraj Hospital, Mahidol University. Objective: To establish a reliable High Performance Liquid Chromatography method with photodiode array detection (HPLC-PDA) and a suitable extraction method for identification and quantification of Aristolochic acid I (AA-I) in 3 different sample types, i.e. Aristolochia tagala Cham.(ATC), Thai Herbal Antitussive 3 Formula (ATF3), and Thai Herbal Antitussive 3 Formula containing ATC (ATF3+ATC). Methods: Two extraction methods, ultrasonication extraction (UE) and solid phase extraction (SPE), were used in the present study. Reversed phase separations were performed with a SunFire C18 column and HPLC-PDA. Mobile phase was performed by 1 mL/min infusion of mixture of 0.1% o-phosphoric acid and methanol (35:65). Results: Calibration curve showed a good linearity with R2 equal to 0.9986. Every sample type has been analyzed. While the concentration of AA-I in ATF3 cannot be determined due to the absence of the AA-I peak, the AA-I concentrations in ATC and ATF3+ATC were 7.50-11.34 and 6.78-11.77 mg/mL respectively. In comparison with UE, SPE is more effective in eradication of the interfering compounds and improves the absorbance spectrum of the peak and increases extraction efficiency. Conclusion: A reliable HPLC-PDA and SPE methods for identification and quantification of AA-I in ATF3+ATC, ATF3, and ATC were established in the present study. The SPE was more effective than UE in the pretreatment step.