Bioaccumulation of heavy metals in water, sediments, aquatic plant and histopathological effects on the golden apple snail in Beung Boraphet reservoir, Thailand

Abstract

Changes in the seasonal concentrations of heavy metals (Cu, Mn, Fe, Zn, Pb and Cd) were determined in water, sediments, snails (Pomacea canaliculata) and aquatic plants (Ipomoea aquatica) in three selected tributaries of the Beung Boraphet reservoir, Nakhon Sawan Province, central Thailand. Only Fe, Cu, Mn and Zn were detected by FAAS in all samples collected. The water quality of Beung Boraphet was medium clean with Fe, Mn, Cu and Zn concentrations well below internationally accepted limits. According to the criteria proposed for sediments by the EPA Region V, Zn and Mn concentrations were within the non-polluted range while Fe and Cu (wet season) concentrations fell into the class of severely polluted sediment. Both P. canaliculata and I. aquatica bioconcentrated more Mn in their tissues than were found in sediments, especially in the wet season. The results of Pearson correlation study and BCF values also indicated similar findings. Only Mn showed the importance of sediment-to-snail concentration and high BCF values in both snails and plants. P. canaliculata exposed to contaminated sediment for two months, showed higher accumulation of metals (Fe, Mn, Cu and Zn) in the digestive tracts and digestive glands than in the foot muscles. Histopathological changes included alterations in the epithelial lining of the digestive tracts, digestive glands and the gills. Loss of cilia and increase in mucous cells were observed in the digestive tracts and gills, while the digestive glands exhibited an increase of dark granules and basophilic cells, and dilation of digestive cells. The results indicated that both P. canaliculata and I. aquatica could be used as biomonitors of sedimentary metal contamination for the Beung Boraphet reservoir. © 2012 Elsevier Inc.