Publication: Cloning and expression of 130-kd mosquito-larvicidal δ-endotoxin gene of Bacillus thuringiensis var. Israelensis in Escherichia coli
3
Issued Date
1987-07-01
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ISSN
16174623
00263925
00263925
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2-s2.0-0023373322
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Mahidol University
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SCOPUS
Bibliographic Citation
MGG Molecular & General Genetics. Vol.208, No.3 (1987), 384-389
Suggested Citation
Chanan Angsuthanasombat, Wipa Chungjatupornchai, Sunee Kertbundit, Plernpis Luxananil, Chatri Settasatian, Prapon Wilairat, Sakol Panyim Cloning and expression of 130-kd mosquito-larvicidal δ-endotoxin gene of Bacillus thuringiensis var. Israelensis in Escherichia coli. MGG Molecular & General Genetics. Vol.208, No.3 (1987), 384-389. doi:10.1007/BF00328128 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/15315
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Title
Cloning and expression of 130-kd mosquito-larvicidal δ-endotoxin gene of Bacillus thuringiensis var. Israelensis in Escherichia coli
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Abstract
Five recombinant E. coli clones exhibiting toxicity to Aedes aegypti larvae were obtained from a library of 800 clones containing XbaI DNA fragments of 110 kb plasmid from B. thuringiensis var. israelensis. All the five clones (pMU 14/258/303/388/679) had the same 3.8-kb insert and encoded a major protein of 130 kDa which was highly toxic to A. aegypti larvae. Three clones (pMU 258/303/388) transcribed the 130 kD a gene in the same direction as that of lac Z promoter of pUC12 vector whereas the transcription of the other two (pMU 14/679) was in the opposite direction. A 1.9-kb fragment of the 3.8 kb insert coded for a protein of 65 kDa. Partial DNA sequence of the 3.8 kb insert, corresponding to the 5′-terminal of the 130 kDa gene, revealed a continuous reading frame, a Shine-Dalgarno sequence and a tentative 5′-regulatory region. These results demonstrated that the 3.8 kb insert is a minimal DNA fragment containing a regulatory region plus the coding sequence of the 130 kDa protein that is highly toxic to mosquito larvae. © 1987 Springer-Verlag.