Publication: Distribution of 28.5 kDa antigen in the tegument of adult Fasciola gigantica
Issued Date
2006-11-01
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ISSN
0001706X
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2-s2.0-33751411906
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Mahidol University
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SCOPUS
Bibliographic Citation
Acta Tropica. Vol.100, No.1-2 (2006), 31-40
Suggested Citation
Panat Anuracpreeda, Chaitip Wanichanon, Kulathida Chaithirayanon, Narin Preyavichyapugdee, Prasert Sobhon Distribution of 28.5 kDa antigen in the tegument of adult Fasciola gigantica. Acta Tropica. Vol.100, No.1-2 (2006), 31-40. doi:10.1016/j.actatropica.2006.09.006 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/23286
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Title
Distribution of 28.5 kDa antigen in the tegument of adult Fasciola gigantica
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Abstract
Monoclonal antibody (MoAb) specific to 28.5 kDa tegumental antigen (TA) was used to localize this antigen in various tissues of adult Fasciola gigantica by means of indirect immunofluorescence, immunoperoxidase and immunogold techniques. The indirect immunofluorescence and immunoperoxidase detections revealed that this antigen was concentrated in the tegument particularly in its outer rim, tegumental cells and their processes, epithelial linings of the oral sucker and the proximal part of digestive tract. It was also detected at a moderate concentration in spermatogenic cells in the testes, cells of Mehlis' gland, oocytes within the ovary, and ovum within the egg of adult parasites. At TEM level, the immunogold detection showed deposit of gold particles specifically in G2tegumental granules and on the surface membrane. Thus, this antigen is expressed in the tegument and associated structures of adult parasites, and it could be a major component of the G2granules which are shown to fuse with the surface membrane and contribute material to replace the casted-off membrane. This process is a part of membrane turnover that prevents the parasite from being attacked by the host immune effector cells. © 2006 Elsevier B.V. All rights reserved.