Publication: Morphometric and molecular differentiation of a Rhodnius robustus-like form from R. robustus Larousse, 1927 and R. prolixus Stal, 1859 (Hemiptera, Reduviidae)
Issued Date
2011-10-01
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ISSN
18736254
0001706X
0001706X
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2-s2.0-80052078361
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Mahidol University
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SCOPUS
Bibliographic Citation
Acta Tropica. Vol.120, No.1-2 (2011), 103-109
Suggested Citation
Edna Márquez, Nicolás Jaramillo-O, Andrés Gómez-Palacio, Jean Pierre Dujardin Morphometric and molecular differentiation of a Rhodnius robustus-like form from R. robustus Larousse, 1927 and R. prolixus Stal, 1859 (Hemiptera, Reduviidae). Acta Tropica. Vol.120, No.1-2 (2011), 103-109. doi:10.1016/j.actatropica.2011.06.009 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/11984
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Title
Morphometric and molecular differentiation of a Rhodnius robustus-like form from R. robustus Larousse, 1927 and R. prolixus Stal, 1859 (Hemiptera, Reduviidae)
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Abstract
In Triatominae, "robustus" group constitutes a cluster of species with great haplotypic divergences but high similarities at morphological and nuclear DNA levels. Given these similarities, species identification generates a frequently problematic issue. In northwestern Amazonia, Rhodnius robustus cohabit with an apparently new species, cryptic with R. robustus (Abad-Franch and Monteiro, 2005). In this region (municipality of Puerto Asís, Department of Putumayo, Colombia), we collected insects classified as R. robustus by traditional keys. We compared this sample with specimens of R. robustus from Venezuela, and of R. prolixus from Colombia and Venezuela. The comparisons used landmark-based geometric morphometrics, and analyses of mitochondrial cytochrome b gene and of D2 variable region of the 28S RNA. The shape of the wings from Puerto Asís specimens disclosed clear-cut divergence from the shape of the wings as found for R. prolixus specimens from Venezuela and Colombia, and diverged from the shape of R. robustus from Venezuela. Thus, morphometric analyses suggested that the Puerto Asís collection could represent a new taxon. Using R. pallescens as an outgroup, a tentative phylogenetic tree based on the geometry of the wing showed the Rhodnius from Puerto Asís more similar to the R. prolixus from Colombia than their congeners from Venezuela. In contrast, the molecular classification clustered Colombian R. prolixus and Venezuelan R. robustus with published GenBank sequences, but it gave the insects from Puerto Asís a basal position to the "robustus" group. This outcome suggests that the Puerto Asís haplotype could be the one found by Abad-Franch and Monteiro (2005). Thus, both morphometric and molecular markers used here, although differing in the phylogenetic classification of samples, could differentiate the Puerto Asís sample from the morphologically similar R. prolixus and R. robustus. This could represent a valuable help in the entomological surveillance related to the control of Chagas disease in the South of Colombia and North of Ecuador. © 2011 Elsevier B.V.