Publication: Stemregenin 1 selectively promotes expansion of multipotent hematopoietic progenitors derived from human embryonic stem cells
Issued Date
2017-01-01
Resource Type
ISSN
09737154
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2-s2.0-85038914094
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Stem Cells and Regenerative Medicine. Vol.13, No.2 (2017), P75-P79
Suggested Citation
Lihong Tao, Padma Priya Togarrati, Kyung Dal Choi, Kran Suknuntha Stemregenin 1 selectively promotes expansion of multipotent hematopoietic progenitors derived from human embryonic stem cells. Journal of Stem Cells and Regenerative Medicine. Vol.13, No.2 (2017), P75-P79. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/42032
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Title
Stemregenin 1 selectively promotes expansion of multipotent hematopoietic progenitors derived from human embryonic stem cells
Abstract
© Journal of Stem Cells and Regenerative Medicine. All rights reserved. Human embryonic stem cell (hESC)-derived hematopoietic stem/progenitor cells hold tremendous potential as alternative cell sources for the treatment of various hematological diseases, drug discovery and toxicological screening. However, limited number of hematopoietic stem/progenitor cells generated from the differentiation of hESCs hinders their downstream applications. Here, we show that aryl hydrocarbon receptor antagonist StemRegenin 1 (SR1) selectively promotes expansion of hESC-derived lin-CD34+hematopoietic progenitors in a concentration-dependent manner. The colony-forming cell (CFC) activity was found to be enriched in the CD34+cells that were expanded with SR1; however, these cells have less colony-forming activity as compared to unexpanded cells (1,338 vs. 7 of CD34+cells to form 1 colony, respectively). Interestingly, SR1 showed a bipotential effect on the proliferation of CD34 negative population, that is low dose of SR1 (1 μM) enhanced cell proliferation, whereas it was repressed at higher doses (>5 μM). In summary, our results suggest that SR1 has the potential to facilitate expansion of hESC-derived lin-CD34+hematopoietic progenitors, which further retain the potential to form multilineage hematopoietic colonies.