Publication: Antioxidant properties of peptides obtained from the split gill mushroom (Schizophyllum commune)
Issued Date
2020-01-01
Resource Type
ISSN
09758402
00221155
00221155
Other identifier(s)
2-s2.0-85086838098
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Journal of Food Science and Technology. (2020)
Suggested Citation
Aunchalee Wongaem, Onrapak Reamtong, Piroonporn Srimongkol, Papassara Sangtanoo, Tanatorn Saisavoey, Aphichart Karnchanatat Antioxidant properties of peptides obtained from the split gill mushroom (Schizophyllum commune). Journal of Food Science and Technology. (2020). doi:10.1007/s13197-020-04582-4 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/57623
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Antioxidant properties of peptides obtained from the split gill mushroom (Schizophyllum commune)
Other Contributor(s)
Abstract
© 2020, Association of Food Scientists & Technologists (India). This study sought to assess the ideal conditions under which hydrolysate can be produced from the split gill mushroom proteins through the microbial protease, Alcalase. The research employed a central composite design and response surface methodology. Three specific parameters were varied for the purposes of the experimental process, while a fixed pH value of 8 was used in all cases. The variables were hydrolysis temperature (set as 45 °C, 50 °C, or 55 °C), hydrolysis time (set as 60 min, 120 min, or 180 min), and the ratio of enzyme to substrate (set as 2%, 4%, or 6% w/v). The variables under investigation exert a significant influence upon degree of hydrolysis (DH) in addition to 2,2′-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical-scavenging activity (p < 0.05). Fractionation of the hydrolysate was accomplished using molecular weight (MW) cut-off membranes, while the greatest radical-scavenging capability was observed in the < 0.65 kDa fraction. The MW < 0.65 kDa fraction underwent separation through RP-HPLC in order to create five sub-fractions. Among these, the greatest ABTS radical-scavenging capability was observed in the F5 sub-fraction, which was therefore chosen to undergo additional examination using quadrupole-time-of-flight-electron spin induction-mass spectrometry-based de novo peptide sequencing. Via this process it was possible to determine five antioxidant peptides. Furthermore, the MW < 0.65 kDa fraction was able to demonstrating cellular antioxidant activity in the context of a human intestinal cancer cell line (HT-29). The extent of this activity was shown to depend upon the concentration levels of the peptide. Graphic abstract: [Figure not available: see fulltext.].