Publication:
Antibody evasion by the P.1 strain of SARS-CoV-2

Issued Date

2021-05-27

Resource Type

Article

ISSN

10974172
00928674

DOI

10.1016/j.cell.2021.03.055

Other identifier(s)

2-s2.0-85104114261

Rights

Mahidol University

Rights Holder(s)

SCOPUS

Bibliographic Citation

Cell. Vol.184, No.11 (2021), 2939-2954.e9

Suggested Citation

Wanwisa Dejnirattisai, Daming Zhou, Piyada Supasa, Chang Liu, Alexander J. Mentzer, Helen M. Ginn, Yuguang Zhao, Helen M.E. Duyvesteyn, Aekkachai Tuekprakhon, Rungtiwa Nutalai, Beibei Wang, César López-Camacho, Jose Slon-Campos, Thomas S. Walter, Donal Skelly, Sue Ann Costa Clemens, Felipe Gomes Naveca, Valdinete Nascimento, Fernanda Nascimento, Cristiano Fernandes da Costa, Paola Cristina Resende, Alex Pauvolid-Correa, Marilda M. Siqueira, Christina Dold, Robert Levin, Tao Dong, Andrew J. Pollard, Julian C. Knight, Derrick Crook, Teresa Lambe, Elizabeth Clutterbuck, Sagida Bibi, Amy Flaxman, Mustapha Bittaye, Sandra Belij-Rammerstorfer, Sarah C. Gilbert, Miles W. Carroll, Paul Klenerman, Eleanor Barnes, Susanna J. Dunachie, Neil G. Paterson, Mark A. Williams, David R. Hall, Ruben J.G. Hulswit, Thomas A. Bowden, Elizabeth E. Fry, Juthathip Mongkolsapaya, Jingshan Ren, David I. Stuart, Gavin R. Screaton Antibody evasion by the P.1 strain of SARS-CoV-2. Cell. Vol.184, No.11 (2021), 2939-2954.e9. doi:10.1016/j.cell.2021.03.055 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/76177

Research Projects

Organizational Units

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Thesis

Title

Antibody evasion by the P.1 strain of SARS-CoV-2

Author(s)

Wanwisa Dejnirattisai
 Daming Zhou
 Piyada Supasa
 Chang Liu
 Alexander J. Mentzer
 Helen M. Ginn
 Yuguang Zhao
 Helen M.E. Duyvesteyn
 Aekkachai Tuekprakhon
 Rungtiwa Nutalai
 Beibei Wang
 César López-Camacho
 Jose Slon-Campos
 Thomas S. Walter
 Donal Skelly
 Sue Ann Costa Clemens
 Felipe Gomes Naveca
 Valdinete Nascimento
 Fernanda Nascimento
 Cristiano Fernandes da Costa
 Paola Cristina Resende
 Alex Pauvolid-Correa
 Marilda M. Siqueira
 Christina Dold
 Robert Levin
 Tao Dong
 Andrew J. Pollard
 Julian C. Knight
 Derrick Crook
 Teresa Lambe
 Elizabeth Clutterbuck
 Sagida Bibi
 Amy Flaxman
 Mustapha Bittaye
 Sandra Belij-Rammerstorfer
 Sarah C. Gilbert
 Miles W. Carroll
 Paul Klenerman
 Eleanor Barnes
 Susanna J. Dunachie
 Neil G. Paterson
 Mark A. Williams
 David R. Hall
 Ruben J.G. Hulswit
 Thomas A. Bowden
 Elizabeth E. Fry
 Juthathip Mongkolsapaya
 Jingshan Ren
 David I. Stuart
 Gavin R. Screaton

Other Contributor(s)

Siriraj Hospital
 NIHR Oxford Biomedical Research Centre
 Oxford University Hospitals NHS Foundation Trust
 Texas A&M College of Veterinary Medicine & Biomedical Sciences
 Public Health England
 Diamond Light Source
 Worthing Hospital
 University of Oxford
 Fundacao Oswaldo Cruz
 Fiocruz Amazônia
 Nuffield Department of Medicine
 University of Oxford Medical Sciences Division
 Instruct-ERIC
 Fundação de Vigilância em Saúde do Amazonas

Abstract

Terminating the SARS-CoV-2 pandemic relies upon pan-global vaccination. Current vaccines elicit neutralizing antibody responses to the virus spike derived from early isolates. However, new strains have emerged with multiple mutations, including P.1 from Brazil, B.1.351 from South Africa, and B.1.1.7 from the UK (12, 10, and 9 changes in the spike, respectively). All have mutations in the ACE2 binding site, with P.1 and B.1.351 having a virtually identical triplet (E484K, K417N/T, and N501Y), which we show confer similar increased affinity for ACE2. We show that, surprisingly, P.1 is significantly less resistant to naturally acquired or vaccine-induced antibody responses than B.1.351, suggesting that changes outside the receptor-binding domain (RBD) impact neutralization. Monoclonal antibody (mAb) 222 neutralizes all three variants despite interacting with two of the ACE2-binding site mutations. We explain this through structural analysis and use the 222 light chain to largely restore neutralization potency to a major class of public antibodies.

Keyword(s)

Biochemistry, Genetics and Molecular Biology

Availability

URI

https://repository.li.mahidol.ac.th/handle/20.500.14594/76177

Collections

Scopus 2021