Publication: Performance of two commercial dengue NS1 rapid tests for the diagnosis of adult patients with dengue infection
Issued Date
2020-01-01
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22288082
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2-s2.0-85079130099
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Mahidol University
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SCOPUS
Bibliographic Citation
Siriraj Medical Journal. Vol.72, No.1 (2020), 74-78
Suggested Citation
Ekkarat Wongsawat, Panisadee Avirutnan, Yoon Won Kim, Yupin Suputtamongkol Performance of two commercial dengue NS1 rapid tests for the diagnosis of adult patients with dengue infection. Siriraj Medical Journal. Vol.72, No.1 (2020), 74-78. doi:10.33192/Smj.2020.10 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/53845
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Title
Performance of two commercial dengue NS1 rapid tests for the diagnosis of adult patients with dengue infection
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Abstract
© 2020 Faculty of Medicine Siriraj Hospital, Mahidol University. Objective: to determine the diagnostic performance of two commercially available dengue NS1 antigen rapid detection tests (RDTs); namely the SD BIOLINE Dengue NS1 RDT and the ImmuneMed Dengue NS1 Rapid, for the diagnosis of adult patients with dengue infection. Methods: The study was performed by using archived samples from 237 patients with the laboratory-confirmed dengue infection. Archived, well-characterized samples from an additional 208 febrile individuals from Thailand were used as the control group. Reference testing to confirm the diagnosis in dengue patients included RT-PCR andin-house NS1 ELISA, in-house IgM and IgG capture ELISAs. Results: The sensitivity of the SD BIOLINE Dengue NS1 RDT was 100%, and the specificity was 99% (95%CI 96.6 to 99.7%). False positive was found in 2 samples from patient with scrub typhus. The sensitivity and specificity of the ImmuneMed Dengue NS1 Ag Rapid were 97.4% (95% CI, 95.5 to 99.5%) and 96.6% (95%CI 93.5 to 98.4%) respectively. False positive results were found in 7 patients with murine typhus, scrub typhus, and influenza. Conclusion: Both RDTs showed comparable sensitivity and specificity in this study population. Data from this study could be used to facilitate data-driven laboratory test choices for managing patient care during dengue outbreaks.