Publication: Effect of synthetic antimicrobial peptides on Naegleria Fowleri trophozoites
Issued Date
2014-05-01
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ISSN
01251562
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2-s2.0-84904573634
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Mahidol University
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SCOPUS
Bibliographic Citation
Southeast Asian Journal of Tropical Medicine and Public Health. Vol.45, No.3 (2014), 537-546
Suggested Citation
Supathra Tiewcharoen, Watchara Phurttikul, Jundee Rabablert, Prasert Auewarakul, Sittiruk Roytrakul, Pruksawan Chetanachan, Thassanant Atithep, Virach Junnu Effect of synthetic antimicrobial peptides on Naegleria Fowleri trophozoites. Southeast Asian Journal of Tropical Medicine and Public Health. Vol.45, No.3 (2014), 537-546. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/34248
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Title
Effect of synthetic antimicrobial peptides on Naegleria Fowleri trophozoites
Abstract
© 2014, SEAMEO TROPMED Network. All Rights Reserved. We evaluated the effect of tritrpticin, lactoferrin, killer decapeptide and scrambled peptide in vitro against Naegleria fowleri trophozoites compared with amphotericin B. Tritrpticin (100 μg/ml) caused apoptosis of N. fowleri trophozoites (2x105cells/ml), while lactoferrin, killer decapeptide and scrambled peptide did not. On Gormori trichrome staining, tritrpticin affected the elasticity of the surface membrane and reduced the size of the nuclei of N. fowleri trophozoites. The ultrastructure surface membrane and food cup formation of the trophozoites were 100% inhibited. These results are consistent with inhibition of the nfa1, Mp2CL5 of the treated trophozoite, which plays a role in food cup formation. Tritrpticin 100 μg/ml was not toxic against SK-N-MC cells. Our findings suggest tritrpticin has activity against the surface membrane and nfa1and Mp2CL5 of N. fowleri trophozoites and could be developed as a potential therapeutic agent.