Publication: Development and validation of an automated solid phase extraction and liquid chromatographic method for the determination of piperaquine in urine
Issued Date
2006-04-11
Resource Type
ISSN
07317085
Other identifier(s)
2-s2.0-33645078879
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Pharmaceutical and Biomedical Analysis. Vol.41, No.1 (2006), 213-218
Suggested Citation
J. Tarning, T. Singtoroj, A. Annerberg, M. Ashton, Y. Bergqvist, N. J. White, N. P J Day, N. Lindegardh Development and validation of an automated solid phase extraction and liquid chromatographic method for the determination of piperaquine in urine. Journal of Pharmaceutical and Biomedical Analysis. Vol.41, No.1 (2006), 213-218. doi:10.1016/j.jpba.2005.10.027 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/23169
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Title
Development and validation of an automated solid phase extraction and liquid chromatographic method for the determination of piperaquine in urine
Abstract
A sensitive and specific bioanalytical method for determination of piperaquine in urine by automated solid-phase extraction (SPE) and liquid chromatography (LC) has been developed and validated. Buffered urine samples (containing internal standard) were loaded onto mixed phase (cation-exchange and octylsilica) SPE columns using an ASPEC XL SPE robot. Chromatographic separation was achieved on a Chromolith Performance RP-18e (100 mm × 4.6 mm I.D.) LC column with phosphate buffer (pH 2.5; 0.1 mol/L)-acetonitrile (92:8, v/v). Piperaquine was analysed at a flow rate of 3 mL/min with UV detection at 347 nm. A linear regression model on log-log transformed data was used for quantification. Within-day precision for piperaquine was 1.3% at 5000 ng/mL and 6.6% at 50 ng/mL. Between-day precision for piperaquine was 3.7% at 5000 ng/mL and 7.2% at 50 ng/mL. Total-assay precision for piperaquine over 4 days using five replicates each day (n = 20) was 4.0%, 5.2% and 9.8% at 5000, 500 and 50 ng/mL, respectively. The lower limit of quantification (LLOQ) was set to 3 ng/mL using 1 mL of urine, which could be lowered to 0.33 ng/mL when using 9 mL of urine and an increased injection volume. © 2005 Elsevier B.V. All rights reserved.