Publication: Cytotoxicity of an experimental light-cured orthodontic adhesive
Issued Date
2019-01-01
Resource Type
ISSN
16629779
Other identifier(s)
2-s2.0-85071870277
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Solid State Phenomena. Vol.294 SSP, (2019), 65-70
Suggested Citation
Kanin Nimcharoensuk, Niwat Anuwongnukroh, Surachai Dechkunakorn, Vanthana Sattabanasuk, Panya Sunintaboon, Wassana Wichai Cytotoxicity of an experimental light-cured orthodontic adhesive. Solid State Phenomena. Vol.294 SSP, (2019), 65-70. doi:10.4028/www.scientific.net/SSP.294.65 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/51182
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Cytotoxicity of an experimental light-cured orthodontic adhesive
Other Contributor(s)
Abstract
© 2019 Trans Tech Publications Ltd, Switzerland. The objective of this study was to compare the cytotoxicity of a domestically-made light-cured orthodontic adhesive to a commercial adhesive, Transbond XT (3M Unitek, USA). An in-house orthodontic adhesive composed of a filler 60-70 weight % and a monomer ratio (BisGMA:TEGDMA) of 6:4 with 0.5% of photoinitiator was mixed. The potential cytotoxic effect of this experimental and a control adhesive was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay according to ISO 10993-5: 2009(E). The L929 cell line was grown in 96-well tissue culture plates (1x105 cells/mm3 ). Thin cured-resin discs of each material weighing 0.4 gram were prepared and incubated for 1, 3, 5, 7, 14, and 30 days in Dulbecco’s modified Eagle medium (DMEM) at 37˚C and 95% humidity with 5% CO2. The percentage of cell viability was reported by descriptive statistics. The result showed that the cell viability of the experimental adhesive was higher than Transbond XT in all measured periods. The cytotoxicity of both the adhesives gradually decreased with the progression of time. In conclusion, the in-house adhesive showed a good biocompatibility since the first day following polymerization. On the other hand, Transbond XT started with a cytotoxic potential, then, turned to be non-cytotoxic after 5 days of curing.