Publication: Evidence for a single active site in β-d-glucosidase/β-d-fucosidase from dalbergia cochinchinensis seeds
Issued Date
1997-01-01
Resource Type
ISSN
13476947
09168451
09168451
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2-s2.0-0030896424
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Mahidol University
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SCOPUS
Bibliographic Citation
Bioscience, Biotechnology and Biochemistry. Vol.61, No.1 (1997), 93-95
Suggested Citation
Rudee Surarit, Hirokazu Matsui, Seiya Chiba, Jisnuson Svasti, Chantragan Srisomsap Evidence for a single active site in β-d-glucosidase/β-d-fucosidase from dalbergia cochinchinensis seeds. Bioscience, Biotechnology and Biochemistry. Vol.61, No.1 (1997), 93-95. doi:10.1271/bbb.61.93 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/17925
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Title
Evidence for a single active site in β-d-glucosidase/β-d-fucosidase from dalbergia cochinchinensis seeds
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Abstract
Kinetic analyses have been done on the hydrolysis of p-nitrophenyl β-d-glucoside (PNPG) and p-nitrophenyl β-d-fucoside (PNPF) by the β-d-glucosidase/ β-d-fucosidase enzyme from Thai Rosewood (Dalbergia cochinchinensis Pierre). PNPF showed a competitive inhibition of PNPG hydrolysis with a Kiof 0.42 mm. Hydrolysis of mixtures of PNPG and PNPF at fractional ratios ranging from 0 to 1 showed Lineweaver–Burk plots intermediate between the two extremes. The apparent Kmand apparent Vmaxat each fractional ratio showed good correspondence with the theoretical curves predicted for the existence of a single common active site for the hydrolysis of the two substrates. © 1997, Taylor & Francis Group, LLC. All rights reserved.