Publication: Viability and morphological changes of Acanthamoeba spp. cysts after treatment with Effective microorganisms (EM)
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Issued Date
2016-06-01
Resource Type
ISSN
09750703
09717196
09717196
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2-s2.0-85028281267
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Parasitic Diseases. Vol.40, No.2 (2016), 369-373
Suggested Citation
Tanitta Sampaotong, Usa Lek-Uthai, Jantima Roongruangchai, Kosol Roongruangchai Viability and morphological changes of Acanthamoeba spp. cysts after treatment with Effective microorganisms (EM). Journal of Parasitic Diseases. Vol.40, No.2 (2016), 369-373. doi:10.1007/s12639-014-0511-x Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/40798
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Title
Viability and morphological changes of Acanthamoeba spp. cysts after treatment with Effective microorganisms (EM)
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Abstract
© 2014, Indian Society for Parasitology. Acanthamoeba is a free-living opportunistic protozoan parasite that is found in diverse environments. It can cause keratitis, mostly related to inappropriate use of contact lenses, as well as life threatening diseases including encephalitis, disseminated sinusitis, and skin ulcers. This study investigated morphological changes and fine structures of the cyst form of Acanthamoeba spp. after treatment with effective microorganisms (EM™) using light and scanning electron microscopies. Acanthamoeba cysts treated with 1:2, 1:4, 1:6, and undiluted EM™ showed higher percentages of non-viable cysts than those treated with 1:8, 1:10, 1:100, 1:200, and 1:400 EM™ and at 5 days post-treatment developed from cystic stage to trophozoite stage. Acanthamoeba cysts treated at concentrations of 1:2, 1:4, 1:6, and undiluted EM™ exhibited cytoplasmic clumping and shrinkage of amoeba cells away from cyst walls. The effective EM™ concentration lethal to Acanthamoeba spp. cyst could provide information to monitor the environmental control system.