Publication: The plasmodium liver-specific protein 2 (LISP2) is an early marker of liver stage development
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Issued Date
2019-05-01
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ISSN
2050084X
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2-s2.0-85067267078
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Mahidol University
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SCOPUS
Bibliographic Citation
eLife. Vol.8, (2019)
Suggested Citation
Devendra Kumar Gupta, Laurent Dembele, Annemarie Voorberg-Van Der Wel, Guglielmo Roma, Andy Yip, Vorada Chuenchob, Niwat Kangwanrangsan, Tomoko Ishino, Ashley M. Vaughan, Stefan H. Kappe, Erika L. Flannery, Jetsumon Sattabongko, Sebastian Mikolajczak, Pablo Bifani, Clemens H.M. Kocken, Thierry Tidiane Diagana The plasmodium liver-specific protein 2 (LISP2) is an early marker of liver stage development. eLife. Vol.8, (2019). doi:10.7554/eLife.43362 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/50196
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Title
The plasmodium liver-specific protein 2 (LISP2) is an early marker of liver stage development
Author(s)
Other Contributor(s)
A-Star, Singapore Immunology Network
Yong Loo Lin School of Medicine
Novartis Institute for Tropical Diseases Pte. Ltd.
Biomedical Primate Research Centre - Rijswijk
Mahidol University
Novartis International AG
Ehime University
Novartis Institute for Tropical Diseases
Center for Infectious Disease Research
Université des Sciences des Techniques et des Technologies de Bamako
Yong Loo Lin School of Medicine
Novartis Institute for Tropical Diseases Pte. Ltd.
Biomedical Primate Research Centre - Rijswijk
Mahidol University
Novartis International AG
Ehime University
Novartis Institute for Tropical Diseases
Center for Infectious Disease Research
Université des Sciences des Techniques et des Technologies de Bamako
Abstract
© Gupta et al. Plasmodium vivax hypnozoites persist in the liver, cause malaria relapse and represent a major challenge to malaria elimination. Our previous transcriptomic study provided a novel molecular framework to enhance our understanding of the hypnozoite biology (Voorberg-van der Wel A, et al., 2017). In this dataset, we identified and characterized the Liver-Specific Protein 2 (LISP2) protein as an early molecular marker of liver stage development. Immunofluorescence analysis of hepatocytes infected with relapsing malaria parasites, in vitro (P. cynomolgi) and in vivo (P. vivax), reveals that LISP2 expression discriminates between dormant hypnozoites and early developing parasites. We further demonstrate that prophylactic drugs selectively kill all LISP2-positive parasites, while LISP2-negative hypnozoites are only sensitive to anti-relapse drug tafenoquine. Our results provide novel biological insights in the initiation of liver stage schizogony and an early marker suitable for the development of drug discovery assays predictive of anti-relapse activity.