Publication: Rapid detection of apolipoprotein E genotypes in Alzheimer's disease using polymerase chain reaction-single strand conformation polymorphism
Issued Date
2006-07-01
Resource Type
ISSN
01251562
Other identifier(s)
2-s2.0-33750215151
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Mahidol University
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SCOPUS
Bibliographic Citation
Southeast Asian Journal of Tropical Medicine and Public Health. Vol.37, No.4 (2006), 793-797
Suggested Citation
Worawan Kamruecha, Sirintorn Chansirikarnjana, Ekapot Nimkulrat, Chesda Udommongkol, Wanna Wongmek, Wipawan Thangnipon Rapid detection of apolipoprotein E genotypes in Alzheimer's disease using polymerase chain reaction-single strand conformation polymorphism. Southeast Asian Journal of Tropical Medicine and Public Health. Vol.37, No.4 (2006), 793-797. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/23714
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Title
Rapid detection of apolipoprotein E genotypes in Alzheimer's disease using polymerase chain reaction-single strand conformation polymorphism
Abstract
Apolipoprotein E (APOE) gene on chromosome 19q13.2 is encoded by three common alleles designated as ε2, ε3 and ε4. In Alzheimer's disease (AD) the ε4 allele is overrepresented and is considered to be a major genetic risk factor. Several methods have been developed to determine/APOEgenotypes. Among them, polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) appears to be highly reliable. In this study, we improved the nonisotopic PCR-SSCP method for determining APOE genotypes in 42 cases of AD patients, 40 cases of non-AD dementia patients, and 49 cases of age-matched controls. DNA from the target sequence on APOE was amplified by PCR from peripheral blood genomic DNA. PCR products were electrophoresed in a non-denaturing polyacrylamide gel and visualized by silver staining. We found that the ε4 allele had a significantly high frequency of occurrence in AD patients (33.3%) compared with age-matched controls (13.3%) (χ2 = 10.43, p = 0.001) and non-AD dementia (10%) (χ2 = 13.02, p<0.001) whereas the ε3 allele was of high frequency in non-AD dementia (90%) compared with age-matched controls (85.7%) and AD patients (66.7%). APOE ε4 homozygotes were found only in AD groups. On the other hand, the ε2 allele was found only in an age-matched control. This study confirmed that the APOE ε4 allele is a risk factor in Thai AD subjects and that the PCR-SSCP method is a rapid and useful means of detecting the APOE genotype in AD.