Publication: Human immune responses to Burkholderia pseudomallei characterized by protein microarray analysis
Issued Date
2011-04-01
Resource Type
ISSN
00221899
Other identifier(s)
2-s2.0-79953019437
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Journal of Infectious Diseases. Vol.203, No.7 (2011), 1002-1011
Suggested Citation
Duangchan Suwannasaen, Jirawan Mahawantung, Wipada Chaowagul, Direk Limmathurotsakul, Philip L. Felgner, Huw Davies, Gregory J. Bancroft, Richard W. Titball, Ganjana Lertmemongkolchai Human immune responses to Burkholderia pseudomallei characterized by protein microarray analysis. Journal of Infectious Diseases. Vol.203, No.7 (2011), 1002-1011. doi:10.1093/infdis/jiq142 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/12577
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Human immune responses to Burkholderia pseudomallei characterized by protein microarray analysis
Abstract
Background. We aimed to determine the antibody and T cell responses to Burkholderia pseudomallei of humans to select candidate vaccine antigens. Methods. For antibody profiling, a protein microarray of 154 B. pseudomallei proteins was probed with plasma from 108 healthy individuals and 72 recovered patients. Blood from 20 of the healthy and 30 of the recovered individuals was also obtained for T cell assays. Results. Twenty-seven proteins distinctively reacted with human plasma following environmental exposure or clinical melioidosis. We compared the responses according to the patient's history of subsequent relapse, and antibody response to BPSL2765 was higher in plasma from individuals who had only 1 episode of disease than in those with recurrent melioidosis. A comparison of antibody and T cell responses to 5 B. pseudomallei proteins revealed that BimA and flagellin-induced responses were similar but that BPSS0530 could induce T cell responses in healthy controls more than in recovered patients. Conclusions. By combining large-scale antibody microarrays and assays of T cell-mediated immunity, we identified a panel of novel B. pseudomallei proteins that show distinct patterns of reactivity in different stages of human melioidosis. These proteins may be useful candidates for development of subunit-based vaccines and in monitoring the risks of treatment failure and relapse. © The Author 2011. Published by Oxford University Press on behalf of the Infectious Diseases Society of America. All rights reserved.