Publication: Thalassemic serum inhibits endothelial cell mitosis in vitro
Issued Date
1997-12-01
Resource Type
ISSN
01251562
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2-s2.0-0031310364
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Mahidol University
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SCOPUS
Bibliographic Citation
Southeast Asian Journal of Tropical Medicine and Public Health. Vol.28, (1997), 155-160
Suggested Citation
Punnee Butthep, Archrob Khuhapinant, Ahnond Bunyaratvej, Suthat Fucharoen, Hiromichi Kitaguchi, Yoshinori Funahara Thalassemic serum inhibits endothelial cell mitosis in vitro. Southeast Asian Journal of Tropical Medicine and Public Health. Vol.28, (1997), 155-160. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/18048
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Title
Thalassemic serum inhibits endothelial cell mitosis in vitro
Abstract
Human umbilical vein endothelial cells were cultured in vitro using Iscove's Modified Dulbecco's Medium (IMDM) supplemented with either pooled normal human serum, or pooled thalassemic serum, or autologous umbilical cord serum, or fetal bovine serum. The mitotic activity was determined under the inverted phase contrast microscope and the number of mitotic cells was counted. Our results showed that the mitotic cells decreased in endothelial cell culture with thalassemic serum as compared with normal human serum, autologous umbilical cord serum or fetal bovine serum. The percentage of mitotic cells decreased on day 3 in the presence of β-thalassemia/HbE serum from both splenectomized and non-splenectomized patients as compared with normal or autologous umbilical cord serum. In the presence of α-thalassemic serum, a similar outcome was also observed. From this study we can conclude that the thalassemic sera might contain factors which affect the endothelial cell growth and proliferation by inhibiting mitosis in vitro.