Publication: On-chip irreversible electroporation for bacterial cell membrane rupture
Issued Date
2012-12-01
Resource Type
Other identifier(s)
2-s2.0-84875086374
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
5th 2012 Biomedical Engineering International Conference, BMEiCON 2012. (2012)
Suggested Citation
S. Jaikla, T. Maturos, T. Pogfay, C. Neatpisarnvanit, P. Sritongkham, A. Tuantranont On-chip irreversible electroporation for bacterial cell membrane rupture. 5th 2012 Biomedical Engineering International Conference, BMEiCON 2012. (2012). doi:10.1109/BMEiCon.2012.6465456 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/14128
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
On-chip irreversible electroporation for bacterial cell membrane rupture
Other Contributor(s)
Abstract
In this work, the DNA preparation process for genetic analysis was developed. To rupture the cell membrane of Salmonella on a microfluidic chip, high electric field pulses were applied through gold electrode. The applied voltage was less than 10V DC voltage. Electric field pulses were short-duration in microsecond. Fluorescent spectrophotometer was employed for live/dead bacterial cells detection. The live bacterial cells were tagged by SYTO 9 green fluorescent stain. The red-fluorescent nucleic acid stain, propidium iodide was used to tag the dead cells. The highest percentage of 95% dead cells was at 7V applied voltage and 90 μs pulse duration. This result was confirmed by plate count with completely dead cell. ©2012 IEEE.