Publication: Heptaphylline induces apoptosis in human colon adenocarcinoma cells through Bid and Akt/NF-κB (p65) pathways
Issued Date
2014-01-01
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ISSN
15137368
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2-s2.0-84921752255
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Mahidol University
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SCOPUS
Bibliographic Citation
Asian Pacific Journal of Cancer Prevention. Vol.15, No.23 (2014), 10483-10487
Suggested Citation
Chantana Boonyarat, Chavi Yenjai, Opa Vajragupta, Pornthip Waiwut Heptaphylline induces apoptosis in human colon adenocarcinoma cells through Bid and Akt/NF-κB (p65) pathways. Asian Pacific Journal of Cancer Prevention. Vol.15, No.23 (2014), 10483-10487. doi:10.7314/APJCP.2014.15.23.10483 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/33427
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Title
Heptaphylline induces apoptosis in human colon adenocarcinoma cells through Bid and Akt/NF-κB (p65) pathways
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Abstract
Heptaphylline derivatives are carbazoles in Clausena harmandiana, a medicinal plant that is utilized for headache, stomach ache, and other treatments of illness. The present study examined the effects of heptaphylline and 7-methoxyheptaphylline on apoptosis of human colon adenocarcinoma cells (HT-29 cell line). Quantification of cell viability was performed using cell proliferation assay (MTT assay) and of protein expression through immunoblotting. The results showed that only heptaphylline, but not 7-methoxyheptaphylline, significantly significantly activated cleaved of caspase-3 and poly (ADP-ribose) polymerase (PARP-1) which resulted in HT-29 cell death. We found that heptaphylline activated BH3 interacting-domain death agonist (Bid) and Bak, proapoptotic proteins. In contrast, it suppressed X-linked inhibitor-of-apoptosis protein (XIAP), Bcl-xL and survivin, inhibitors of apoptosis. In addition, heptaphylline inhibited activation of NF-κB/p65 (rel), a regulator of apoptotic regulating proteins by suppressing the activation of Akt and IKKα, upstream regulators of p65. The findings suggested that heptaphylline induces apoptosis in human colon adenocarcinoma cells .