Publication: Fasciola gigantica cathepsin B5 is an acidic endo- and exopeptidase of the immature and mature parasite
Issued Date
2015-12-01
Resource Type
ISSN
61831638
03009084
03009084
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2-s2.0-84944075407
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Mahidol University
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SCOPUS
Bibliographic Citation
Biochimie. Vol.119, (2015), 6-15
Suggested Citation
Sinee Siricoon, Suksiri Vichasri Grams, Kittisak Lertwongvisarn, Muntana Abdullohfakeeyah, Peter M. Smooker, Rudi Grams Fasciola gigantica cathepsin B5 is an acidic endo- and exopeptidase of the immature and mature parasite. Biochimie. Vol.119, (2015), 6-15. doi:10.1016/j.biochi.2015.10.005 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/35337
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Title
Fasciola gigantica cathepsin B5 is an acidic endo- and exopeptidase of the immature and mature parasite
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Abstract
© 2015 Elsevier B.V. and Société Française de Biochimie et Biologie Moléculaire (SFBBM). All rights reserved. Cysteine proteases of the liver fluke Fasciola have been described as essential molecules in the infection process of the mammalian host. Destinct cathepsin Bs, which are already expressed in the metacercarial stage and released by the newly excysted juvenile are major actors in this process. Following infection their expression is stopped and the proteins will not be detectable any longer after the first month of development. On the contrary, the novel cathepsin B5 of Fasciola gigantica (FgCB5) described in this work was also found expressed in later juvenile stages and the mature worm. Like all previously described Fasciola family members it was located in the cecal epithelium of the parasite. Western blot analysis of adult antigen preparations detected procathepsin B5 in crude worm extract and in small amounts in the ES product. In support of these data, the sera of infected rabbits and mice were reactive with recombinant FgCB5 in Western blot and ELISA. Biochemical analysis of yeast-expressed FgCB5 revealed that it has properties of a lysosomal hydrolase optimized for activity at acid pH and that it is able to efficiently digest a broad spectrum of host proteins. Unlike previously characterized Fasciola family members FgCB5 carries a histidine doublet in the occluding loop equivalent to residues His110 and His111 of human mature cathepsin B and consequently showed substantial carboxydipeptidyl activity which depends on these two residues.