Publication: Rapid detection of dengue viral RNA by nucleic acid sequence-based amplification (NASBA)
Issued Date
2002-12-01
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ISSN
1020895X
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2-s2.0-0141829212
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Mahidol University
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SCOPUS
Bibliographic Citation
Dengue Bulletin. Vol.26, (2002), 125-130
Suggested Citation
Wipawee Usawattanakul, Akanitt Jittmittraphap, Timothy P. Endy, Ananda Nisalak, Pramuan Tapchaisri, Sornchai Looareesuwan Rapid detection of dengue viral RNA by nucleic acid sequence-based amplification (NASBA). Dengue Bulletin. Vol.26, (2002), 125-130. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/20177
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Title
Rapid detection of dengue viral RNA by nucleic acid sequence-based amplification (NASBA)
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Abstract
The suitability of RNA amplification by nucleic acid sequence-based amplification (NASBA) for the detection of dengue viral RNA was investigated. A set of primers and probe were synthesized, based on a selected RNA sequence from the non-coding region at the 3′ end of dengue viral RNA, and was used in the NASBA assay. The NASBA reaction product was then determined by agarose gel electrophoresis and electrochemiluminescence (ECL) signal count. The sensitivity of the NASBA assay was equal to 1 PFU/ml for all four dengue virus serotypes. There was no false positive result with Japanese encephalitis (JE) virus. This method was used successfully to detect dengue virus in the infected tissue culture cells. This test will be useful for the detection of dengue viruses in the clinical specimens.