Publication:
Crystallization and preliminary crystallographic study of the functional form of the Bacillus thuringiensis mosquito-larvicidal Cry4Aa mutant toxin

Issued Date

2004-07-01

Resource Type

Article

ISSN

09074449

DOI

10.1107/S0907444904011205

Other identifier(s)

2-s2.0-15744373506

Rights

Mahidol University

Rights Holder(s)

SCOPUS

Bibliographic Citation

Acta Crystallographica Section D: Biological Crystallography. Vol.60, No.7 (2004), 1315-1318

Suggested Citation

Panadda Boonserm, Chanan Angsuthanasombat, Julien Lescar Crystallization and preliminary crystallographic study of the functional form of the Bacillus thuringiensis mosquito-larvicidal Cry4Aa mutant toxin. Acta Crystallographica Section D: Biological Crystallography. Vol.60, No.7 (2004), 1315-1318. doi:10.1107/S0907444904011205 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/21175

Research Projects

Organizational Units

Authors

Journal Issue

Thesis

Title

Crystallization and preliminary crystallographic study of the functional form of the Bacillus thuringiensis mosquito-larvicidal Cry4Aa mutant toxin

Author(s)

Panadda Boonserm
 Chanan Angsuthanasombat
 Julien Lescar

Other Contributor(s)

Mahidol University
 Nanyang Technological University

Abstract

The 65 kDa functional form of the mosquito-larvicidal Cry4Aa-R235Q mutant toxin has been crystallized. The crystals belong to space group C222 1, with unit-cell parameters a = 91.2, b = 202.1, c = 98.7 Å, and contain one molecule per asymmetric unit. The crystals diffract to ~∼.9 Å using synchrotron radiation and a complete native data set has been collected. The structure has been solved using a molecular-replacement method with the Cry4Ba toxin protein as a search model. © 2004 International Union of Crystallography.

Keyword(s)

Biochemistry, Genetics and Molecular Biology
 Physics and Astronomy

Availability

URI

https://repository.li.mahidol.ac.th/handle/20.500.14594/21175

Collections

Scopus 2001-2005