Publication: Development of monoclonal antibody-based enzyme-linked immunosorbent assay for quantitative quality control of Derris scandens (Roxb.) Benth
Issued Date
2019-07-04
Resource Type
ISSN
15324230
15321819
15321819
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2-s2.0-85065833659
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Immunoassay and Immunochemistry. Vol.40, No.4 (2019), 407-418
Suggested Citation
Kamonthip Jutathis, Benyakan Pongkitwitoon, Boonchoo Sritularak, Hiroyuki Tanaka, Waraporn Putalun Development of monoclonal antibody-based enzyme-linked immunosorbent assay for quantitative quality control of Derris scandens (Roxb.) Benth. Journal of Immunoassay and Immunochemistry. Vol.40, No.4 (2019), 407-418. doi:10.1080/15321819.2019.1615942 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/50129
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Title
Development of monoclonal antibody-based enzyme-linked immunosorbent assay for quantitative quality control of Derris scandens (Roxb.) Benth
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Abstract
© 2019, © 2019 Taylor & Francis. Derris scandens (Roxb.) Benth. is a medicinal plant used for treatment of musculoskeletal pain in Thai traditional medicines. Its stem contains active compound genistein-7-O-[α-rhamnopyranosyl-(1 to 6)-β-glucopyranoside] (GTG) which is used as a biomarker for standardization of D. scandens extracts. As an alternative for rapid quantitation of GTG, a monoclonal antibody against GTG was prepared and applied for an indirect competitive enzyme-linked immunosorbent assay (ELISA) to determine GTG in plants and herbal products. The established method provided a quantification range of 0.31–10 µg/mL with a limit of detection of 0.29 µg/mL. The assay was validated for precision and accuracy by intra- and interassay variation analyses, recovery test, and comparison analysis between the amounts of GTG determined by ELISA and HPLC. The results exhibited that the developed ELISA is sensitive and effective for determination of GTG in D. scandens plant materials and herbal products.