Publication: PRMT1-mediated methylation of MICU1 determines the UCP2/3 dependency of mitochondrial Ca<sup>2+</sup> uptake in immortalized cells
Issued Date
2016-09-19
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ISSN
20411723
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2-s2.0-84988474788
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Mahidol University
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SCOPUS
Bibliographic Citation
Nature Communications. Vol.7, (2016)
Suggested Citation
Corina T. Madreiter-Sokolowski, Christiane Klec, Warisara Parichatikanond, Sarah Stryeck, Benjamin Gottschalk, Sergio Pulido, Rene Rost, Emrah Eroglu, Nicole A. Hofmann, Alexander I. Bondarenko, Tobias Madl, Markus Waldeck-Weiermair, Roland Malli, Wolfgang F. Graier PRMT1-mediated methylation of MICU1 determines the UCP2/3 dependency of mitochondrial Ca<sup>2+</sup> uptake in immortalized cells. Nature Communications. Vol.7, (2016). doi:10.1038/ncomms12897 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/42973
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Title
PRMT1-mediated methylation of MICU1 determines the UCP2/3 dependency of mitochondrial Ca<sup>2+</sup> uptake in immortalized cells
Abstract
© 2016 The Author(s). Recent studies revealed that mitochondrial Ca2+ channels, which control energy flow, cell signalling and death, are macromolecular complexes that basically consist of the pore-forming mitochondrial Ca2+ uniporter (MCU) protein, the essential MCU regulator (EMRE), and the mitochondrial Ca2+ uptake 1 (MICU1). MICU1 is a regulatory subunit that shields mitochondria from Ca2+ overload. Before the identification of these core elements, the novel uncoupling proteins 2 and 3 (UCP2/3) have been shown to be fundamental for mitochondrial Ca2+ uptake. Here we clarify the molecular mechanism that determines the UCP2/3 dependency of mitochondrial Ca2+ uptake. Our data demonstrate that mitochondrial Ca2+ uptake is controlled by protein arginine methyl transferase 1 (PRMT1) that asymmetrically methylates MICU1, resulting in decreased Ca2+ sensitivity. UCP2/3 normalize Ca2+ sensitivity of methylated MICU1 and, thus, re-establish mitochondrial Ca2+ uptake activity. These data provide novel insights in the complex regulation of the mitochondrial Ca2+ uniporter by PRMT1 and UCP2/3.