Publication:
Label-free fluorescent detection of loop-mediated isothermal amplification of nucleic acid using pyrophosphate-selective xanthene-based Zn(II)-coordination chemosensor

Issued Date

2012-12-01

Resource Type

Article

ISSN

13480715
03667022

DOI

10.1246/cl.2012.1666

Other identifier(s)

2-s2.0-84872230875

Rights

Mahidol University

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SCOPUS

Bibliographic Citation

Chemistry Letters. Vol.41, No.12 (2012), 1666-1668

Suggested Citation

Ekkachai Kittiloespaisan, Akio Ojida, Itaru Hamachi, Yortyot Seetang-Nun, Wansika Kiatpathomchai, Jirarut Wongkongkatep Label-free fluorescent detection of loop-mediated isothermal amplification of nucleic acid using pyrophosphate-selective xanthene-based Zn(II)-coordination chemosensor. Chemistry Letters. Vol.41, No.12 (2012), 1666-1668. doi:10.1246/cl.2012.1666 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/13932

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Title

Label-free fluorescent detection of loop-mediated isothermal amplification of nucleic acid using pyrophosphate-selective xanthene-based Zn(II)-coordination chemosensor

Author(s)

Ekkachai Kittiloespaisan
 Akio Ojida
 Itaru Hamachi
 Yortyot Seetang-Nun
 Wansika Kiatpathomchai
 Jirarut Wongkongkatep

Other Contributor(s)

Mahidol University
 Kyushu University
 Kyoto University
 Thailand National Center for Genetic Engineering and Biotechnology

Abstract

A label-free fluorescent detection method for the loopmediated isothermal amplification (LAMP) of nucleic acids was first developed by using the pyrophosphate-selective xanthenebased Zn(II)-coordination chemosensor 12Zn(II). In this method, the production of inorganic pyrophosphate (PPi), a by-product of the LAMP reaction, was detected by 12Zn(II) with a large fluorescence enhancement. On combination with the fluorescent plate reader technique, this method allows us to detect the shrimp white spot syndrome virus in a highthroughput manner with high sensitivity and selectivity. © 2012 The Chemical Society of Japan.

Keyword(s)

Chemistry

Availability

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https://repository.li.mahidol.ac.th/handle/20.500.14594/13932

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