Publication: Acute interactions between intestinal sugar and calcium transport in vitro
Issued Date
2014-01-01
Resource Type
ISSN
15221547
01931857
01931857
Other identifier(s)
2-s2.0-84891506090
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
American Journal of Physiology - Gastrointestinal and Liver Physiology. Vol.306, No.1 (2014)
Suggested Citation
Phuntila Tharabenjasin, Veronique Douard, Chirag Patel, Nateetip Krishnamra, Richard J. Johnson, Jian Zuo, Ronaldo P. Ferraris Acute interactions between intestinal sugar and calcium transport in vitro. American Journal of Physiology - Gastrointestinal and Liver Physiology. Vol.306, No.1 (2014). doi:10.1152/ajpgi.00263.2013 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/33490
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Acute interactions between intestinal sugar and calcium transport in vitro
Abstract
Fructose consumption by Americans has increased markedly, whereas Ca2+intake has decreased below recommended levels. Because fructose metabolism decreases enterocyte ATP concentrations, we tested the hypothesis that luminal fructose acutely reduces active, diet-inducible Ca2+transport in the small intestine. We confirmed that the decrease in ATP concentrations was indeed greater in fructose- compared with glucose-incubated mucosal homogenates from wild-type and was prevented in fructose-incubated homogenates from ketohexokinase (KHK)-/-mice. We then induced active Ca2+transport by chronically feeding wild-type, fructose transporter glucose transporter 5 (GLUT5)-/-, as well as KHK-/-mice a low Ca2+diet and measured transepithelial Ca2+transport in everted duodenal sacs incubated in solutions containing glucose, fructose, or their nonmetabolizable analogs. The diet-induced increase in active Ca2+transport was proportional to dramatic increases in expression of the Ca2+-selective channel transient receptor potential vanilloid family calcium channel 6 as well as of the Ca2+-binding protein 9k (CaBP9k) but not that of the voltage-dependent L-type channel Ca(v)1.3. Crypt-villus distribution of CaBP9k seems heterogeneous, but low Ca2+diets induce expression in more cells. In contrast, KHK distribution is homogeneous, suggesting that fructose metabolism can occur in all enterocytes. Diet-induced Ca2+transport was not enhanced by addition of the enterocyte fuel glutamine and was always greater in sacs of wild-type, GLUT5-/-, and KHK-/-mice incubated with fructose or nonmetabolizable sugars than those incubated with glucose. Thus duodenal Ca2+transport is not affected by fructose and enterocyte ATP concentrations but instead may decrease with glucose metabolism, as Ca2+transport remains high with 3-O-methylglucose that is also transported by sodium-glucose cotransporter 1 but cannot be metabolized. © 2014 the American Physiological Society.