Publication:
Isothiocyanatoluminol as a chemiluminescence labeling reagent for amino acids and proteins

Issued Date

2011-02-04

Resource Type

Article

ISSN

13480715
03667022

DOI

10.1246/cl.2011.188

Other identifier(s)

2-s2.0-79551471336

Rights

Mahidol University

Rights Holder(s)

SCOPUS

Bibliographic Citation

Chemistry Letters. Vol.40, No.2 (2011), 188-190

Suggested Citation

Srung Smanmoo, Weerachai Nasomphan, Pramuan Tangboriboonrat Isothiocyanatoluminol as a chemiluminescence labeling reagent for amino acids and proteins. Chemistry Letters. Vol.40, No.2 (2011), 188-190. doi:10.1246/cl.2011.188 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/11729

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Title

Isothiocyanatoluminol as a chemiluminescence labeling reagent for amino acids and proteins

Author(s)

Srung Smanmoo
 Weerachai Nasomphan
 Pramuan Tangboriboonrat

Other Contributor(s)

Thailand National Center for Genetic Engineering and Biotechnology
 Mahidol University

Abstract

Isothiocyanatoluminol (ITL) was successfully synthesized from the reaction between luminol and thiophosgene in the presence of triethylamine. The optimized conditions were extended for the preparation of isothiocyanatoisoluminol (ITIL) and phenyl isothiocyanate (PI). The chemiluminescence (CL) of ITL was comparable to luminol. ITL was used as a CL labeling reagent for amino acid and bovine serum albumin (BSA) in which the -NH 2 terminus of amino acid residue present in BSA acts as the labeling site. © 2011 The Chemical Society of Japan.

Keyword(s)

Chemistry

Availability

URI

https://repository.li.mahidol.ac.th/handle/20.500.14594/11729

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Scopus 2011-2015