Publication: Vagus nerve stimulation even after injury ameliorates cisplatin-induced nephropathy via reducing macrophage infiltration
Issued Date
2020-12-01
Resource Type
ISSN
20452322
Other identifier(s)
2-s2.0-85086320063
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Scientific Reports. Vol.10, No.1 (2020)
Suggested Citation
Rie Uni, Tsuyoshi Inoue, Yasuna Nakamura, Daichi Fukaya, Sho Hasegawa, Chia Hsien Wu, Rie Fujii, Bongkod Surattichaiyakul, Wachirasek Peerapanyasut, Atsuko Ozeki, Nobuyoshi Akimitsu, Youichiro Wada, Masaomi Nangaku, Reiko Inagi Vagus nerve stimulation even after injury ameliorates cisplatin-induced nephropathy via reducing macrophage infiltration. Scientific Reports. Vol.10, No.1 (2020). doi:10.1038/s41598-020-66295-0 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/58397
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Vagus nerve stimulation even after injury ameliorates cisplatin-induced nephropathy via reducing macrophage infiltration
Abstract
© 2020, The Author(s). The efficacy of prior activation of an anti-inflammatory pathway called the cholinergic anti-inflammatory pathway (CAP) through vagus nerve stimulation (VNS) has been reported in renal ischemia-reperfusion injury models. However, there have been no reports that have demonstrated the effectiveness of VNS after injury. We investigated the renoprotective effect of VNS in a cisplatin-induced nephropathy model. C57BL/6 mice were injected with cisplatin, and VNS was conducted 24 hours later. Kidney function, histology, and a kidney injury marker (Kim-1) were evaluated 72 hours after cisplatin administration. To further explore the role of the spleen and splenic macrophages, key players in the CAP, splenectomy, and adoptive transfer of macrophages treated with the selective α7 nicotinic acetylcholine receptor agonist GTS-21 were conducted. VNS treatment significantly suppressed cisplatin-induced kidney injury. This effect was abolished by splenectomy, while adoptive transfer of GTS-21-treated macrophages improved renal outcomes. VNS also reduced the expression of cytokines and chemokines, including CCL2, which is a potent chemokine attracting monocytes/macrophages, accompanied by a decline in the number of infiltrating macrophages. Taken together, stimulation of the CAP protected the kidney even after injury in a cisplatin-induced nephropathy model. Considering the feasibility and anti-inflammatory effects of VNS, the findings suggest that VNS may be a promising therapeutic tool for acute kidney injury.