Publication: Copper ions potentiate organic hydroperoxide and hydrogen peroxide toxicity through different mechanisms in Xanthomonas campestris pv. campestris
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2010-12-01
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15746968
03781097
03781097
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2-s2.0-78349260817
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Mahidol University
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SCOPUS
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FEMS Microbiology Letters. Vol.313, No.1 (2010), 75-80
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Nisa Patikarnmonthon, Sirikan Nawapan, Sarinya Buranajitpakorn, Nisanart Charoenlap, Skorn Mongkolsuk, Paiboon Vattanaviboon Copper ions potentiate organic hydroperoxide and hydrogen peroxide toxicity through different mechanisms in Xanthomonas campestris pv. campestris. FEMS Microbiology Letters. Vol.313, No.1 (2010), 75-80. doi:10.1111/j.1574-6968.2010.02124.x Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/28585
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Copper ions potentiate organic hydroperoxide and hydrogen peroxide toxicity through different mechanisms in Xanthomonas campestris pv. campestris
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Copper (Cu)-based biocides are important chemical controls for both fungal and bacterial diseases in crop fields. Here, we showed that Cu ions at a concentration of 100 μM enhanced t-butyl hydroperoxide (tBOOH) and hydrogen peroxide (H2O2) killing of Xanthomonas campestris pv. campestris through different mechanisms. The addition of an antilipid peroxidation agent (α-tocopherol) and hydroxyl radical scavengers (glycerol and dimethyl sulphoxide) partially protected the bacteria from the Cu-enhanced tBOOH and H2O2killing, respectively. Inactivation of the alkyl hydroperoxide reductase gene rendered the mutant vulnerable to lethal doses of copper sulphate, which could be alleviated by the addition of an H2O2scavenger (pyruvate) and α-tocopherol. Taken together, the data suggest that Cu ions influence the killing effect of tBOOH through the stimulation of lipid peroxidation, while hydroxyl radical production is the underlying mechanism responsible for the Cu-ion-enhanced H2O2killing effects. © 2010 Federation of European Microbiological Societies.